【摘要】：干旱脅迫對(duì)植物生長(zhǎng)發(fā)育造成嚴(yán)重的損傷。小麥作為一種主要的糧食作物,如何有效緩解其在干旱脅迫下所受損傷,是解決人們生存大計(jì)的重要課題。三唑類化合物具有植物生長(zhǎng)調(diào)節(jié)作用,同時(shí)可有效緩解非生物脅迫等對(duì)植物體的迫害。本文通過(guò)觀察己唑醇硝酸銅配合物對(duì)植物氣孔和根的形態(tài)影響,以及干旱脅迫下配合物對(duì)小麥幼苗ABA含量及 ABA 相關(guān)基因(ABA 合成關(guān)鍵基因 9-cis-epoxycarotenoid dioxigenase(jNCED)和ABA分解重要基因ABA8'-hydroxylase(CYP707A1))表達(dá)的影響,,探其是否可以緩解脅迫對(duì)植物造成的損害,提高植物體的耐旱性。(1)小麥和煙草葉片下表皮組織,進(jìn)行不同濃度配合物溶液和不同時(shí)間處理。0-100 μM配合物處理1h,隨著配合物濃度增加,煙草和小麥氣孔開度逐漸減小,100μM時(shí)兩者氣孔開度達(dá)到最小值。設(shè)定的6個(gè)濃度(0μM、20μM、40μM、60μM、80μM、100 μM)下,煙草氣孔開度平均值從2.37 μm減小到0.90 μm。而小麥氣孔開度平均值大小為:1.06 μm、0.96 μm、0.75 μm、0.63 μm、0.59 μm、0.55 μm。0-9h 不同時(shí)間配合物處理下,煙草和小麥氣孔孔徑同樣隨著處理時(shí)間的延長(zhǎng),逐漸減小。煙草氣孔孔徑平均值在80 μM配合物處理后,從0 h的2.37 μm減小到9 h的0.94 μm。小麥經(jīng)60 μM配合物處理,氣孔孔徑相比對(duì)照依次減小40.6%(1 h)、49.1%(3 h)、50.9%(6 h)、54.7%(9 h)。己唑醇硝酸銅配合物可降低植物氣孔孔徑。掃描電鏡觀察小麥根尖在60 μ己唑醇硝酸銅配合物處理下的變化。對(duì)照組小麥根尖根毛稀疏、短粗。處理組小麥根尖根毛濃密、纖長(zhǎng)。兩組根尖形態(tài)學(xué)上端無(wú)明顯差別,而從距根尖形態(tài)學(xué)上端5mm處開始處理組根毛相比對(duì)照組濃密、纖長(zhǎng)。(2)利用實(shí)時(shí)定量PCR(real-time quantitative PCR,RT-qPCR)方法探究己唑醇硝酸銅配合物在干旱脅迫下對(duì)小麥幼苗ABA含量和相關(guān)基因NCED和CYP7ED和表達(dá)量的影響。結(jié)果顯示,干旱狀態(tài)下,對(duì)照組和己唑醇硝酸銅配合物處理組NCED表達(dá)量均低于未處理0h時(shí)的表達(dá)量。但在失水過(guò)程中(24-144h)對(duì)照組表達(dá)量逐漸升高,而處理組NCED表達(dá)量先降低(24-48 h)后升高(96-144 h);復(fù)水后(144-194 h)兩組表達(dá)量均下調(diào)表達(dá)。ABA分解關(guān)鍵基因CYP707A1表達(dá)量在整個(gè)處理過(guò)程中,相對(duì)0 h時(shí)都有所增高,對(duì)照組在144 h達(dá)到最高,處理組168 h表達(dá)量最高。同樣,對(duì)照組和處理組CYP707A1表達(dá)趨勢(shì)不同,對(duì)照組表達(dá)量在干旱階段均高于處理組,復(fù)水后卻低于處理組。實(shí)驗(yàn)發(fā)現(xiàn),CYP707A1表達(dá)量的變化大于NCED。脅迫下,小麥幼苗葉片對(duì)照組和處理組ABA含量相對(duì)Oh都升高,96 h達(dá)到最大值,分別為Oh的4.38倍、4.64倍。整個(gè)過(guò)程中,處理組ABA高于對(duì)照組,所以,配合物具有提高小麥幼苗干旱耐受效能(3)通過(guò)對(duì)CYP707A1基因表達(dá)量的檢測(cè),驗(yàn)證了結(jié)合PCR擴(kuò)增技術(shù)及金納米星/石墨烯/沉積納米金復(fù)合材料制備的電化學(xué)生物傳感器的測(cè)定性能。結(jié)果表明,使用生物傳感器檢測(cè)的上節(jié)中不同處理小麥CYP707A1基因表達(dá)量與傳統(tǒng)的RT-qPCR結(jié)果一致。并且,該生物傳感器的檢測(cè)范圍寬至5個(gè)數(shù)量級(jí),最低檢測(cè)可達(dá)到13 pM,顯示電化學(xué)生物傳感器具有好的靈敏性、特異性。
[Abstract]:Drought stress causes serious damage to plant growth and development. As a major food crop, how to effectively alleviate the damage caused by drought stress in wheat is an important issue to solve people's livelihood. In this paper, the effects of copper hexazole nitrate complex on the stomatal and root morphology of plants, ABA content and ABA related genes (9-cis-epoxycarotenoid dioxigenase (jNCED) and ABA decomposition important genes (CYP707A1) expression in Wheat Seedlings under drought stress were studied. (1) The stomatal openings of tobacco and wheat decreased gradually with the increase of the concentration of the complexes, and reached to 100 mu M when the lower epidermal tissues of wheat and tobacco leaves were treated with different concentrations of complex solution and different time. The average stomatal aperture of tobacco and wheat decreased from 2.37 micron to 0.90 micron at 6 concentrations (0, 20, 40, 60, 80, 100 micron). The average stomatal aperture of wheat was 1.06 micron, 0.96 micron, 0.75 micron, 0.63 micron, 0.59 micron, 0.55 micron, 0.0-9 h, respectively. The average stomatal diameter of tobacco decreased from 2.37 micron in 0 h to 0.94 micron in 9 h after treatment with 80 micron complexes. The stomatal diameter of wheat treated with 60 micron complexes decreased by 40.6% (1 h), 49.1% (3 h), 50.9% (6 h), 54.7% (9 h) compared with the control in turn. The root hairs of the control group were thinner, shorter and thicker than those of the control group. The root hairs of the treatment group were dense and slender. There was no significant difference between the two groups in morphology, but the root hairs of the treatment group were denser and slender than those of the control group from 5 mm away from the top. Real-time quantitative PCR (RT-qPCR) was used to investigate the effects of copper hexazole nitrate complex on ABA content, NCED and CYP7ED expression in Wheat Seedlings under drought stress. However, during dehydration (24-144h), the expression of NCED in the control group increased gradually, and the expression of NCED in the treatment group decreased first (24-48h) and then increased (96-144h); after rehydration (144-194h), the expression of CYP707A1 in both groups was down-regulated. Similarly, the expression of CYP707A1 in control group and treatment group was higher than that in treatment group at drought stage, but lower than that in treatment group after rehydration. The change of expression of CYP707A1 was greater than that in NCED. Under stress, the ABA content in control group and treatment group of wheat seedling leaves increased relative to Oh. In the whole process, the ABA of treatment group was higher than that of control group, so the complex could improve drought tolerance of wheat seedlings (3) The electrochemical synthesis of Au nano-star/graphene/deposited Au nanocomposites was verified by detecting the expression of CYP707A1 gene. The results showed that the expression of CYP707A1 gene in wheat treated with different treatments in the previous section detected by biosensor was consistent with the results of traditional RT-q PCR. Specificity.
【學(xué)位授予單位】：西北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q943.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孔欣欣;周炳森;;土壤、稻桿和稻米中己唑醇?xì)埩袅康臋z測(cè)方法[J];生物技術(shù)世界;2012年06期

2 莊漢平,冉崇英,盧家爛;有機(jī)—銅配合物熱穩(wěn)定性及熱分解產(chǎn)物的實(shí)驗(yàn)研究[J];現(xiàn)代地質(zhì);1996年03期

3 蘇占華;王春梅;趙志鳳;顧佳;周百斌;;2,6-吡啶二羧酸銅配合物的合成與晶體結(jié)構(gòu)分析[J];哈爾濱師范大學(xué)自然科學(xué)學(xué)報(bào);2011年01期

4 沈孟長(zhǎng);陳曉青;羅勤慧;;若干銅配合物歧化超氧離子的活性[J];生物化學(xué)與生物物理進(jìn)展;1987年04期

5 陳中方,潘蔭明,馬克勤,郭蔚輝,郝策,趙學(xué)莊,唐敖慶;水溶性C_(60)賴氨酸衍生物及其銅配合物的合成與表征[J];南開大學(xué)學(xué)報(bào)(自然科學(xué)版);1998年02期

6 夏玉明,樸惠善,羅惠善;槲皮素銅配合物的合成及其活性研究[J];延邊大學(xué)學(xué)報(bào)(自然科學(xué)版);2000年01期

7 李寶龍;二個(gè)新的銅配合物的合成,結(jié)構(gòu)和性質(zhì)的研究[J];蘇州大學(xué)學(xué)報(bào)(自然科學(xué));2002年02期

8 劉瓊;李海平;徐小燕;黃鳳仙;陳希;王明召;;銅配合物的分子幾何結(jié)構(gòu)模擬方法研究[J];北京師范大學(xué)學(xué)報(bào)(自然科學(xué)版);2007年05期

9 郭秀英，，王鳳山;甘二肽·咪唑合銅配合物的合成、晶體結(jié)構(gòu)和分子結(jié)構(gòu)的研究[J];內(nèi)蒙古民族師院學(xué)報(bào)(自然科學(xué)版);1996年02期

10 李智念,王光明,曾之文;植物干旱脅迫中的ABA研究[J];干旱地區(qū)農(nóng)業(yè)研究;2003年02期

相關(guān)會(huì)議論文 前10條

1 彭亞軍;王彥輝;李欣;周小毛;柏連陽(yáng);;5%己唑醇懸浮劑在水稻中的殘留及消解動(dòng)態(tài)[A];公共植保與綠色防控[C];2010年

2 李曦;肖紅玉;劉鵬;張超燦;王成剛;;銅配合物對(duì)大腸桿菌生長(zhǎng)代謝的微量熱研究[A];中國(guó)化學(xué)會(huì)第十三屆全國(guó)化學(xué)熱力學(xué)和熱分析學(xué)術(shù)會(huì)議論文摘要集[C];2006年

3 劉清霞;李阿惠;劉寶林;陶偌偈;;雙核銅配合物的合成和晶體結(jié)構(gòu)[A];河南省化學(xué)會(huì)2012年學(xué)術(shù)年會(huì)論文摘要集[C];2012年

4 呂壘;李大成;竇建民;;2-吡啶乙醇銅配合物的合成及結(jié)構(gòu)[A];大環(huán)化學(xué)和超分子化學(xué)的新發(fā)展——當(dāng)前學(xué)科交叉的一個(gè)重要橋梁——中國(guó)化學(xué)會(huì)全國(guó)第十五屆大環(huán)化學(xué)暨第七屆超分子化學(xué)學(xué)術(shù)討論會(huì)論文摘要集[C];2010年

5 薛美香;劉世雄;;一個(gè)具有一維孔洞的雙核銅配合物的合成、晶體結(jié)構(gòu)與表征[A];中國(guó)晶體學(xué)會(huì)第四屆全國(guó)會(huì)員代表大會(huì)暨學(xué)術(shù)會(huì)議學(xué)術(shù)論文摘要集[C];2008年

6 劉建軍;黃長(zhǎng)滄;陳建中;;兩個(gè)二酰胺基硫脲衍生物的八核銅配合物[A];中國(guó)化學(xué)會(huì)2013年中西部地區(qū)無(wú)機(jī)化學(xué)化工學(xué)術(shù)研討會(huì)論文集[C];2013年

7 邱曉;蔣勇;萬(wàn)重慶;李夏;;銅配合物的合成和結(jié)構(gòu)[A];第七屆全國(guó)催化劑制備科學(xué)與技術(shù)研討會(huì)論文集[C];2009年

8 張慶富;張海娜;張

本文編號(hào)：2179417