【摘要】：為探討牛樟芝子實體和不同培養(yǎng)時期的菌絲體中三萜含量及鯊烯環(huán)氧酶基因(Se)和甲羥戊酸焦磷酸脫羧酶基因(Mvd)的表達情況,采用香草醛-冰醋酸法測定菌絲體和子實體的三萜含量,并利用實時熒光定量PCR(qRT-PCR)分析甲羥戊酸途徑(MVA)中Se和Mvd基因表達水平。結(jié)果表明,隨著培養(yǎng)時間的推移,當(dāng)生長至21 d時牛樟芝菌絲體干重逐漸趨于穩(wěn)定。當(dāng)培養(yǎng)時間小于28 d時,菌絲體中三萜含量隨著培養(yǎng)時間的延長而逐漸增加,且28 d時菌絲體的三萜含量最高,達(39.192±2.025)mg/g,僅次于牛樟芝子實體三萜含量(49.391±2.675)mg/g,玉米芯栽培的牛樟芝子實體三萜含量最低,為(11.530±0.733)mg/g,各樣品間三萜含量差異達到顯著水平(P0.05)。qRT-PCR分析結(jié)果表明,培養(yǎng)7 d的菌絲體中Se和Mvd的表達量均為最高,但隨著培養(yǎng)時間的繼續(xù)延長而呈現(xiàn)下降趨勢;牛樟芝子實體中Se和Mvd的表達量均較低,與培養(yǎng)28~42 d的菌絲體表達量相當(dāng)。本研究結(jié)果表明牛樟芝的三萜含量可能與其合成途徑中相關(guān)基因的表達量、培養(yǎng)條件和累積時間有關(guān)。
[Abstract]:In order to study the triterpene content in the fruiting body and mycelium of Coyoderma lucidum and the expression of squalene epoxylase gene (Se) and (Mvd) in the mycelium of different culture period, The triterpenoid content of mycelium and fruiting body was determined by vanillin-glacial acetic acid method. The expression of se and Mvd genes in (MVA) via mevalic acid pathway was analyzed by real-time fluorescence quantitative PCR (qRT-PCR). The results showed that the dry weight of mycelium tended to be stable at 21 days with the passage of culture time. When the culture time was less than 28 days, the triterpenoid content of mycelia increased with the extension of culture time, and the triterpenoid content of mycelium was the highest at 28 days. The content of triterpenoids was (39.192 鹵2.025) mg / g, which was only (49.391 鹵2.675) mg / g in the fruiting body of Camphor lucidum, and the lowest (11.530 鹵0.733) mg / g in corncob. The difference of triterpene content among the samples was significant (P0.05) .qRT-PCR analysis showed that the content of triterpenoid in the fruiting body of the corncob was (11.530 鹵0.733) mg / g. The expression of se and Mvd was the highest in mycelia cultured for 7 days, but decreased with the prolongation of culture time, and the expression of se and Mvd in the fruiting body of Bos camphora was lower than that of the mycelia cultured for 2842 days. The results suggested that the triterpenoid content of Acanthus camphora might be related to the expression of related genes in its synthesis pathway, culture conditions and accumulation time.
【作者單位】： 國家菌草工程技術(shù)研究中心;福建農(nóng)林大學(xué)生命科學(xué)學(xué)院;福建省農(nóng)業(yè)科學(xué)院果樹研究所;
【基金】：福建省菌草生態(tài)產(chǎn)業(yè)協(xié)同創(chuàng)新中心(K80ND8002) 福建農(nóng)林大學(xué)科技發(fā)展基金(KF2015111) 國家菌草工程技術(shù)研究中心組建項目(2011FU125X12)
【分類號】：S567.3

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