【摘要】：本研究旨在探討限飼對湖羊子宮RGMb基因及BMP系統(tǒng)成員表達的影響。首先應(yīng)用qPCR檢測RGMb mRNA在3月齡湖羊機體的組織表達譜,應(yīng)用IHC檢測RGMb在子宮中的定位。然后選取妊娠35d湖羊分為兩組,對照組(C)飼喂100%NRC日糧,限飼組(R)飼喂50%NRC日糧,妊娠110天取其子宮組織(n=8),應(yīng)用qPCR和Western blotting(WB)檢測BMP2、BMP4、BMPR1A、BMPR1B、BMPR2與RGMb的表達。結(jié)果,RGMb mRNA在湖羊機體的14種組織中均表達,且在子宮的相對表達量大于0.5。湖羊子宮RGMb主要定位于腔上皮與腺上皮細胞。RGMb蛋白在限飼組(R)湖羊子宮中的表達較對照組(C)顯著下降(P0.05),但mRNA水平則無顯著變化(P0.05);限飼組(R)湖羊子宮中的BMP2與BMP4 mRNA水平較對照組(C)顯著上升(P0.05),而BMP受體BMPR1A、BMPR1B與BMPR2mRNA水平較對照組顯著下降(P0.05)。限飼影響子宮組織BMP系統(tǒng)成員尤其是RGMb蛋白的表達,提示RGMb可能參與BMP系統(tǒng)對湖羊子宮穩(wěn)態(tài)的調(diào)控,為進一步研究BMP在子宮中的作用機制提供了試驗依據(jù)。
[Abstract]:The purpose of this study was to investigate the effects of feeding restriction on the expression of RGMb gene and BMP system members in uterus of Hu sheep. QPCR was used to detect the tissue expression profile of RGMb mRNA in 3 months old Hu sheep, and IHC was used to detect the localization of RGMb in uterus. After 35 days of pregnancy, Hu sheep were divided into two groups. The control group was fed with 100%NRC diet, the control group was fed with 50%NRC diet, and the uterine tissue (nb8) was collected on the 110th day of gestation. The expression of BMPR1A, BMPR1BU BMPR2 and RGMb in BMP2BMP4BMP4, BMPR1B, BMPR2 and RGMb were detected by qPCR and Western blotting (WB). Results RGMb mRNA was expressed in 14 kinds of tissues of Hu sheep, and the relative expression of RGMb mRNA in uterus was more than 0.5. The expression of RGMb protein in uterine epithelium and glandular epithelium of Hu sheep was significantly lower than that in control group (P0.05), but the level of mRNA had no significant change (P0.05), and BMP2 and BMP4 mRNA in the uterus of (R) Hu sheep were not significantly changed (P0.05), and the expression of RGMb protein in the uterus of (R) Hu sheep was significantly lower than that of control group (P0.05). Compared with the control group, the level of (C) increased significantly (P0.05), while the BMP receptor BMPR1 Agna BMPR1B and BMPR2mRNA levels decreased significantly compared with the control group (P0.05). The effects of feeding restriction on the expression of BMP protein, especially RGMb protein in uterine tissues, suggested that RGMb might be involved in the regulation of uterine homeostasis by BMP system in Hu sheep, which provided the experimental basis for further study on the mechanism of BMP in uterus.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動物科技學(xué)院;江蘇省肉羊產(chǎn)業(yè)工程技術(shù)研究中心;啟東瑞鵬牧業(yè)有限公司;
【基金】：江蘇省農(nóng)業(yè)科技支撐計劃(BE2014330)
【分類號】：S826

【相似文獻】

相關(guān)期刊論文 前10條

1 張炳坤;早期限飼對肉仔雞的影響[J];中國飼料;2005年07期

2 呂玲;;限飼對雞的行為模式影響較小[J];中國家禽;2013年19期

3 王吉;任鳳麗;;不同限飼方法對種雞經(jīng)濟效益的影響[J];遼寧畜牧獸醫(yī);1992年02期

4 石天虹;肉仔雞生長早期不同限飼方式對生長補償和胴體成分的影響[J];山東家禽;1994年03期

5 閆興才;長周期飼養(yǎng)肉仔雞用限飼技術(shù)效果好[J];當(dāng)代畜牧;2001年06期

6 馬振平,曹翠菊;蛋雞限飼能降耗增效[J];河北畜牧獸醫(yī);2001年02期

7 孫祥海,孫懷剛,王永軍,孫軍;種雞生長期的限飼及注意的事項[J];黑龍江畜牧獸醫(yī);2002年05期

8 李玉欣,咼于明;早期限飼對肉仔雞蛋白質(zhì)周轉(zhuǎn)代謝的影響[J];中國農(nóng)業(yè)大學(xué)學(xué)報;2003年03期

9 李玉欣,咼于明,曹兵海,袁建敏,聶偉;生長早期不同能量、蛋白限飼水平對肉仔雞補償生長的影響[J];中國畜牧雜志;2003年03期

10 鐔龍;限飼對肉雞的影響[J];中國家禽;2005年06期

相關(guān)會議論文 前10條

1 莊君英;許雪萍;夏東;楊曉靜;倪迎冬;李曉;趙茹茜;;早期限飼對肉雞脂質(zhì)過氧化和抗氧化酶活性的長期影響[A];全國動物生理生化第九次學(xué)術(shù)交流會論文摘要匯編[C];2006年

2 莊君英;夏東;楊曉靜;許雪萍;倪迎冬;李曉;趙茹茜;;早期限飼對肉雞脂質(zhì)過氧化和抗氧化酶活性長期影響的觀察[A];全國動物生理生化第十次學(xué)術(shù)交流會論文摘要匯編[C];2008年

3 許雪萍;楊曉靜;倪迎冬;夏東;李曉;趙茹茜;;早期限飼對肉雞肌肉生長的程序化影響[A];全國動物生理生化第九次學(xué)術(shù)交流會論文摘要匯編[C];2006年

4 趙高;黃艷群;陳文;王佳偉;韓瑞麗;孫桂榮;田亞東;康相濤;;限飼對肉雞生長及屠體性狀的影響[A];中國家禽業(yè)——機遇與挑戰(zhàn)——第十三次全國家禽學(xué)術(shù)討論會論文集[C];2007年

5 李s，

本文編號：2152365