發(fā)布時間：2018-07-25 12:43

【摘要】：本研究首次從仿刺參(Apostichopus japonicus)體壁中克隆得到核糖體蛋白L30(ribosomal protein L30,RPL30)的c DNA全長序列(Gen Bank:JQ770165),該序列包括56 bp的5′-UTR,162 bp的3′-UTR和339b p的開放閱讀框,共編碼112個氨基酸;Blast比對分析結(jié)果顯示該序列的核苷酸序列以及推導的氨基酸序列與其他物種的同源性均在75%以上;RPL30基因在仿刺參各組織中均有表達,其中腸、體腔細胞、縱肌、體壁中表達量分別為呼吸樹的7.24倍(P0.01)、4.47倍(P0.01)、3.12倍(P0.05)、1.35倍(P0.05);仿刺參體壁再生不同階段核糖體蛋白基因RPL30和RPL17的表達存在差異,體壁再生第7 d時RPL30基因的表達出現(xiàn)峰值,為對照組的2.13倍(P0.05),其余天數(shù)的相對表達量均低于對照組,其中第1、5、6d的表達量與對照組差異均顯著(P0.05);RPL17基因在再生第2、5d分別出現(xiàn)峰值,分別為對照組的7.47倍和5.60倍(P0.05),其余各天的表達量與對照組差異不顯著(P0.05)。研究結(jié)果表明核糖體蛋白基因除構(gòu)成核糖體參與蛋白質(zhì)合成外,還有著各自的核糖體外功能。本研究結(jié)果將為進一步研究仿刺參蛋白質(zhì)合成、再生及多種生理活動的調(diào)控機制奠定基礎(chǔ)。
[Abstract]:In this study, the full-length DNA sequence (Gen Bank:JQ770165) of ribosomal protein L30 (ribosomal protein L30RPL30 was first cloned from the body wall of (Apostichopus japonicus). The sequence consists of 56 BP 3'-UTR and 339bp open reading frame. The results of cocoding 112 amino acid blast analysis showed that the nucleotide sequence of the sequence and the deduced amino acid sequence had more than 75% homology with other species. The RPL30 gene was expressed in all tissues, including intestinal and somatic cells. The expression of ribosomal protein gene RPL30 and RPL17 in longitudinal muscle and body wall were 7.24 times (P0.01) 4.47 times (P0.01) 3.12 times (P0.05) and 1.35 times (P0.05) of respiratory tree, respectively, the expression of ribosomal protein gene RPL30 and RPL17 were different in different stages of body wall regeneration, and the peak expression of RPL30 gene appeared at the 7th day of wall regeneration. The relative expression of RPL17 gene in the control group was 2.13 times of that in the control group (P0.05), and the relative expression of RPL17 gene in the other days was lower than that in the control group. There were significant differences in the expression of RPL17 gene between the control group and the control group on the 6th day (P0.05) and the peak value of RPL17 gene on the 2nd day of regeneration. It was 7.47 times and 5.60 times of the control group respectively (P0.05). The expression of the other days was not significantly different from that of the control group (P0.05). The results showed that ribosomal protein genes had their own ribosomal functions in addition to ribosomes involved in protein synthesis. The results of this study will lay a foundation for the further study of protein synthesis, regeneration and regulation mechanism of various physiological activities of Acanthopsis japonicus.
【作者單位】： 大連海洋大學遼寧省海洋生物資源與生境修復重點實驗室;大連海洋大學農(nóng)業(yè)部北方海水增養(yǎng)殖重點實驗室;
【基金】：國家自然科學基金(30371099) 遼寧省教育廳創(chuàng)新團隊(2007T015);遼寧省教育廳實驗室專項(2008S064)
【分類號】：S917.4

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