【摘要】：為了闡明藏雞尼克酰胺磷酸核糖轉(zhuǎn)移酶(NAMPT)基因的組織表達(dá)譜,并分析NAMPT基因表達(dá)水平與肌內(nèi)脂肪含量的相關(guān)關(guān)系,試驗(yàn)利用索氏抽提法測定各器官組織中肌內(nèi)脂肪含量,通過熒光定量PCR檢測技術(shù)測定NAMPT基因在藏雞不同器官組織中的表達(dá)情況,并與肌內(nèi)脂肪含量進(jìn)行相關(guān)分析。結(jié)果表明:NAMPT基因在藏雞脂肪組織中表達(dá)水平最高,極顯著高于其他組織(P0.01)。NAMPT基因在胸肌和腿肌中的表達(dá)無顯著的性別差異。NAMPT mRNA表達(dá)量與藏雞胸肌和腿肌肌內(nèi)脂肪含量相關(guān)關(guān)系呈性別分化現(xiàn)象,在公雞中呈正相關(guān)關(guān)系,在母雞中呈負(fù)相關(guān)關(guān)系,NAMPT基因可能與藏雞生長發(fā)育過程中的肌內(nèi)脂肪沉積有關(guān)。
[Abstract]:In order to elucidate the tissue expression profile of nicotinamide phosphate ribonucleosyltransferase (NAMPT) gene in Tibetan chicken, and analyze the relationship between the expression level of NAMPT gene and the content of intramuscular fat, Soxhlet extraction method was used to determine the content of intramuscular fat in various organs and tissues. The expression of NAMPT gene in different organs and tissues of Tibetan chicken was detected by fluorescence quantitative PCR, and the correlation between the expression of NAMPT gene and the content of intramuscular fat was analyzed. The results showed that the highest expression level of the gene was found in the adipose tissue of Tibetan chicken. The expression of NAMPT gene was significantly higher than that of other tissues (P0.01). There was no significant gender difference in the expression of NAMPT gene in the pectoralis and leg muscles. There was a positive correlation between the expression of NAMPT mRNA and the fat content in the pectoralis and leg muscles of Tibetan chickens. There was a negative correlation between NAMPT gene and intramuscular fat deposition during the growth and development of Tibetan chickens.
【作者單位】： 四川廣播電視大學(xué)農(nóng)林衛(wèi)生學(xué)院;西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)基金項(xiàng)目“藏雞肌苷酸相關(guān)基因表達(dá)研究及其調(diào)控網(wǎng)絡(luò)的構(gòu)建”(2015NZYQN58) 四川省科技支撐計劃項(xiàng)目“肉雞現(xiàn)代產(chǎn)業(yè)鏈關(guān)鍵技術(shù)集成研究與產(chǎn)業(yè)化示范”(2016NZ0003)
【分類號】：S831

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