【摘要】：為了對山東省濱州市周邊地區(qū)6株新城疫流行情況進(jìn)行研究,試驗對不同毒株的治病力進(jìn)行測定,采用RT-PCR技術(shù)擴(kuò)增F基因,利用Meg Align軟件進(jìn)行序列分析,同時克隆DZNDV/007株F蛋白的兩個抗原表位F72和F161,構(gòu)建兩個融合表達(dá)質(zhì)粒p ET-32a-F72和p ET-32a-F161,并對其進(jìn)行誘導(dǎo)表達(dá)及Western-blot分析。結(jié)果表明:5株屬于基因Ⅱ型,為弱毒株;1株屬于基因Ⅶ型,為強(qiáng)毒株;IPTG誘導(dǎo)得到兩個大量表達(dá)的融合蛋白His-F72和His-F161,且具有良好的反應(yīng)原性。說明新城疫基因Ⅶ型毒株已成為我國的主要致病毒株。
[Abstract]:In order to study the epidemic situation of 6 strains of Newcastle disease in the surrounding area of Binzhou City, Shandong Province, the therapeutic power of different strains was tested. The F gene was amplified by RT-PCR and sequenced by Meg Align software. At the same time, two antigenic epitopes F72 and F161of F protein of DZNDV / 007 strain were cloned, two fusion expression plasmids pET-32a-F72 and pET-32a-F161were constructed, and their expression was induced and analyzed by Western-blot. The results showed that the fusion proteins His-F72 and His-F161were induced by IPTG of virulent strain and virulent strain, respectively, and had good reactivity. The results showed that 5 strains belonged to gene type 鈪，

本文編號：2135462