【摘要】：為了對山東省濱州市周邊地區(qū)6株新城疫流行情況進行研究,試驗對不同毒株的治病力進行測定,采用RT-PCR技術擴增F基因,利用Meg Align軟件進行序列分析,同時克隆DZNDV/007株F蛋白的兩個抗原表位F72和F161,構建兩個融合表達質粒p ET-32a-F72和p ET-32a-F161,并對其進行誘導表達及Western-blot分析。結果表明:5株屬于基因Ⅱ型,為弱毒株;1株屬于基因Ⅶ型,為強毒株;IPTG誘導得到兩個大量表達的融合蛋白His-F72和His-F161,且具有良好的反應原性。說明新城疫基因Ⅶ型毒株已成為我國的主要致病毒株。
[Abstract]:In order to study the epidemic situation of 6 strains of Newcastle disease in the surrounding area of Binzhou City, Shandong Province, the therapeutic power of different strains was tested. The F gene was amplified by RT-PCR and sequenced by Meg Align software. At the same time, two antigenic epitopes F72 and F161of F protein of DZNDV / 007 strain were cloned, two fusion expression plasmids pET-32a-F72 and pET-32a-F161were constructed, and their expression was induced and analyzed by Western-blot. The results showed that the fusion proteins His-F72 and His-F161were induced by IPTG of virulent strain and virulent strain, respectively, and had good reactivity. The results showed that 5 strains belonged to gene type 鈪，

本文編號：2135462