【摘要】：近年來環(huán)介導(dǎo)等溫擴(kuò)增技術(shù)(Loop-Mediated Isothermal Amplification,LAMP)越來越多地應(yīng)用于轉(zhuǎn)基因農(nóng)作物、微生物等的基因檢測中。為了提高相關(guān)檢測機(jī)構(gòu)的轉(zhuǎn)基因產(chǎn)品檢測效率,加快進(jìn)出口食品農(nóng)產(chǎn)品的通關(guān)速度,本文首次使用碟式芯片環(huán)介導(dǎo)恒溫擴(kuò)增技術(shù),在65℃恒溫條件下,對樣品中的P-Ca MV35S、T-NOS、NPTⅡ、BAR、PAT、Cry1Ac、EPSPS I、EPSPS II、FMV35S和GM-HRA等轉(zhuǎn)基因靶標(biāo)進(jìn)行同步檢測,檢出限可達(dá)到0.5%(m/m),實(shí)現(xiàn)一次上樣即可完成樣品的轉(zhuǎn)基因成分篩查。該方法特異性和靈敏度良好、檢測時間較短,可實(shí)時讀取擴(kuò)增結(jié)果。對實(shí)際樣品的檢測結(jié)果表明,該方法的檢測結(jié)果與實(shí)時熒光PCR方法的完全一致。本文開發(fā)的轉(zhuǎn)基因檢測微流控恒溫擴(kuò)增碟式芯片,可用于常見農(nóng)產(chǎn)品(大豆、玉米、大米和棉花等)的轉(zhuǎn)基因成分篩查,為轉(zhuǎn)基因產(chǎn)品的定性檢測提供了新的快速、靈敏、高通量技術(shù)平臺。
[Abstract]:In recent years, Loop-Mediated Isothermal Amplification (lamp) has been used more and more in gene detection of transgenic crops and microbes. In order to improve the detection efficiency of transgenic products and speed up the customs clearance of imported and exported food and agricultural products, this paper first used disc chip ring mediated thermostat amplification technology, at 65 鈩，

本文編號：2119446