【摘要】：采用PCR-SSCP技術(shù)對45頭種公牛的ACTB(β肌動蛋白)基因的第4外顯子的單核甘酸多態(tài)性進行了檢測。結(jié)果顯示:在ACTB基因第4外顯子上存在2種基因型,即AA型和AB型。對該多肽片段克隆測序,結(jié)果在ACTB基因第4外顯子上存在G378C突變。用最小二乘法分析ACTB基因的第4外顯子不同基因型與精液品質(zhì)的相關(guān)性,發(fā)現(xiàn)ACTB基因第4外顯子AA型精子濃度、鮮精畸形率以及凍精畸形率顯著高于AB型(P0.05),AA型鮮精活力、鮮精活精子比率極顯著高于AB型(P0.01),AA型凍精活精子比率顯著低于AB型(P0.05)。
[Abstract]:The mononuclear glycosylated polymorphism of exon 4 of ACTB (尾 actin) gene in 45 cattle was detected by PCR-SSCP. The results showed that there were two genotypes on exon 4 of ACTB gene, namely AA type and AB type. The polypeptide fragment was cloned and sequenced. G378C mutation was found in exon 4 of ACTB gene. The correlation between different genotypes of exon 4 of ACTB gene and semen quality was analyzed by least square method. It was found that the sperm concentration, deformity rate of fresh spermatozoa and deformity rate of frozen sperm in exon 4 of ACTB gene were significantly higher than those of type AB (P0.05). The percentage of fresh spermatozoa was significantly higher than that of type AB (P0.01) and that of type AA was significantly lower than that of type AB (P0.05).
【作者單位】： 遵義醫(yī)學(xué)院珠海校區(qū);吉林大學(xué)實驗動物中心;涿州市動物衛(wèi)生監(jiān)督所;吉林省長春優(yōu)質(zhì)肉牛繁育中心;
【基金】：肉牛牦牛產(chǎn)業(yè)技術(shù)體系資助項目(CARS-38) 國家科技支撐計劃資助項目(2011BAD19B04)
【分類號】：S823

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