本文選題：MATE轉(zhuǎn)運蛋白 + ELS1��； 參考：《蘭州大學(xué)》2017年碩士論文

【摘要】：多藥和有毒化合物排出蛋白(MATE,multidrug and toxic compound extrusion)廣泛存在于生物界,能夠作為次級轉(zhuǎn)運系統(tǒng)的一部分對細胞內(nèi)的化學(xué)離子或其它小分子物質(zhì)濃度進行調(diào)節(jié),以應(yīng)對環(huán)境的變化。鐵是植物中重要的微量營養(yǎng)元素之一,體內(nèi)鐵元素的穩(wěn)態(tài)調(diào)節(jié)對于植物的生長發(fā)育來說非常重要。在擬南芥的至少58個MATE基因中,只有2個被證明參與了鐵穩(wěn)態(tài)調(diào)節(jié)。本實驗室之前對激活標(biāo)簽篩選得到的突變體els1-D(Early Leaf Senescence 1)的研究發(fā)現(xiàn),ELS1編碼一個MATE蛋白,其過量異位表達能夠?qū)е氯~片的早衰,說明MATE蛋白ELS1可能參與衰老過程中營養(yǎng)物質(zhì)的再分配。但對于它的更進一步認識有待研究,如它具體如何參與此過程、表達模式及調(diào)控方式、亞細胞定位以及是否存在其它轉(zhuǎn)運底物等。此外,由于過表達突變體有很多問題不容易解釋,所以本論文為更進一步了解ELS1基因的生理功能,著重對其T-DNA插入缺失突變體els1-1進行了研究。通過觀察不同時期的植株表型發(fā)現(xiàn)缺失突變體els1-1與野生型并無明顯差別;通過鐵染色檢測不同時期幼苗體內(nèi)鐵含量發(fā)現(xiàn)缺失突變體els1-1中鐵含量多于野生型;對缺失突變體els1-1和野生型進行黑暗誘導(dǎo)實驗,發(fā)現(xiàn)缺失突變體els1-1衰老晚于野生型;而且對正常條件下生長植株外源施加鐵,能延緩els1-D的衰老;對pELS1::GUS轉(zhuǎn)基因植株進行鐵處理后GUS染色發(fā)現(xiàn)鐵能夠誘導(dǎo)該基因表達;此外,亞細胞分析發(fā)現(xiàn)ELS1定位于細胞的內(nèi)膜系統(tǒng)。綜合以上結(jié)果,我們推測ELS1可能定位在擬南芥的液泡膜上,參與將細胞內(nèi)多余的鐵離子運輸?shù)揭号葜?所以當(dāng)ELS1缺失以后將打破細胞內(nèi)鐵離子的穩(wěn)態(tài)平衡。但目前對于ELS1參與的植物鐵穩(wěn)態(tài)調(diào)控中的其它參與因子及詳細的調(diào)控機制我們還知之甚少,所以需要我們更深入的研究。本研究將拓展我們對植物MATE家族基因功能的認識,有可能為農(nóng)作物的改良提供理論依據(jù)和思路。
[Abstract]:Multidrug and toxic compound extrusion) is widely present in the biological world and can regulate the concentration of chemical ions or other small molecules in cells as part of the secondary transport system in order to cope with environmental changes. Iron is one of the most important micronutrients in plants. Of at least 58 MATE genes in Arabidopsis thaliana, only 2 have been shown to be involved in iron homeostasis regulation. Previous studies in our laboratory on the mutant els1-D (early Leaf Senescence 1) screened for activation tags have found that ESL _ 1 encodes a mate protein, and its over-ectopic expression can lead to premature senescence in leaves. These results suggest that ELS1 may be involved in the redistribution of nutrients during senescence. However, further understanding of it remains to be studied, such as how it participates in this process, how it is expressed and regulated, its subcellular localization and the existence of other transport substrates. In addition, there are many problems in overexpression mutants which are difficult to explain. In order to understand the physiological function of ELS1 gene, the T-DNA insertion deletion mutant els1-1 was studied in this paper. It was found that there was no significant difference between the deletion mutant els1-1 and the wild type by observing the plant phenotypes at different stages, and the iron content in the deletion mutant els1-1 was higher than that in the wild type by iron staining. The dark induction experiments of els1-1 and wild-type mutant showed that els1-1 was older than wild type, and exogenous iron applied to the growth plants under normal conditions could delay the senescence of Els 1-D. Gus staining of pELS1: Gus transgenic plants showed that iron could induce the expression of the gene, and that ELS1 was located in the endomembrane system of the cells by subcellular analysis. Based on the above results, we speculate that ELS1 may be located on the vacuolar membrane of Arabidopsis thaliana and be involved in the transport of excess iron ions into vacuoles. Therefore, the homeostasis of iron ions in the cells will be broken when ELS1 is absent. However, we still know very little about the other factors and detailed regulation mechanism of plant iron homeostasis regulated by ELS1, so we need to study more deeply. This study will expand our understanding of the gene function of plant MATE family and may provide theoretical basis and ideas for crop improvement.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：Q943.2

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本文編號：2118589