本文選題：褐飛虱 + 脂代謝��； 參考：《揚州大學》2016年碩士論文

【摘要】：褐飛虱Nilaparvata lugens (Stal)是亞洲地區(qū)一種遠距離遷飛性的水稻害蟲。褐飛虱再猖獗涉及到毒物興奮效應及補償作用、農(nóng)藥刺激害蟲生殖的生理機制和藥劑刺激害蟲生殖的分子機制等。本文嘗試通過對褐飛虱脂肪體蛋白質分析和RNA干擾(RNA interference, RNAi)方法研究三唑磷對褐飛虱脂代謝及生殖的影響。1.兩種藥劑處理的褐飛虱脂肪體脂質代謝分析在昆蟲的生長發(fā)育、生殖過程中,脂質代謝起著重要的作用,脂肪體是褐飛虱的代謝中心。本實驗使用蛋白質組學分析方法,研究了三唑磷和茚蟲威處理后褐飛虱脂肪體中脂質代謝的變化。共檢測出91個脂質代謝相關的差異蛋白,占注釋到具體通路中差異蛋白的18.1%,其中重要的有胰脂肪酶甘油三酯、谷氨酰胺合成酶、乙酰膽堿酯酶、過氧化物酶�；o酶A氧化酶等。共19條顯著差異的脂質代謝通路,占差異通路的8.5%,包括脂肪的消化和吸收、脂肪酸代謝等其他脂類代謝途徑。重要的有不飽和脂肪酸的生物合成、甘油脂代謝、甘油脂代謝、脂肪酸延長、脂肪的消化和吸收、甘油磷脂代謝、類固醇激素的生物合成、磷酸肌醇代謝等。由此可見,三唑磷處理的褐飛虱脂肪體中,脂類的代謝有著顯著的變化。2.干擾�；o酶A氧化酶基因對褐飛虱生殖的影響本試驗分析研究了各種處理的褐飛虱的差異蛋白,結果顯示三唑磷顯著刺激了脂類代謝的�；o酶A氧化酶(Acyl-coenzyme A Oxidase, ACO)表達。在隨后的RNAi實驗中發(fā)現(xiàn),取食含有dsACO人工飼料的褐飛虱的生殖力顯著下降。TZP+dsACO處理與TZP和TZP+dsGFP相比,產(chǎn)卵量分別下降了30%和32%；產(chǎn)卵歷期分別下降了24%和21%；雌成蟲壽命分別下降了20%和26%。褐飛虱雌成蟲體內(nèi)粗脂肪也顯著減少,TZP+dsACO處理與TZP相比,粗脂肪含量顯著下降42%(從5.3mg到3.1mg)。褐飛虱體內(nèi)各種脂肪酸含量也有不同程度的變化,TZP+dsACO處理組褐飛虱雌蟲脂肪體中油酸、棕櫚酸、亞油酸、硬脂酸、肉豆蔻酸、油酸、棕櫚酸、肉豆蔻酸含量與對照相比均顯著下降。除此之外,TZP+dsACO處理與TZP相比,蛋白質含量顯著下降11.8%(從197ug/五頭雌蟲到174ug/五頭雌蟲)。表明ACO是TZP誘導刺激BPH生殖的基因之一。
[Abstract]:The Nilaparvata lugens (Stal) of brown planthopper (Stal) is a long-range migratory pest of rice in Asia. The re rampant of brown planthopper involves the effect of toxicant excitatory and compensation, the physiological mechanism of pesticide stimulation to the reproduction of pests and the molecular mechanism of the insecticide to stimulate the reproduction of the pest. This article attempts to analyze the protein of the fat body of the brown planthopper and the RNA dry. RNA interference (RNAi) method study the effect of three zazophos on lipid metabolism and reproduction of brown planthopper. The lipid metabolism analysis of the fat body of brown planthopper, treated by.1. two agents, plays an important role in the growth and development of the insects. The lipid body is the metabolic center of the brown planthopper. The changes in lipid metabolism in the fat bodies of three zazophos and ninophos were studied. 91 differential proteins related to lipid metabolism were detected, accounting for 18.1% of the differential proteins in the specific pathway, including triglyceride, glutamine synthase, acetylcholinesterase and peroxidase A. A total of 19 significant differences in lipid metabolic pathways, accounting for 8.5% of the differential pathways, including fat digestion and absorption, fatty acid metabolism, and other lipid metabolism pathways. The biosynthesis of unsaturated fatty acids, triglyceride metabolism, triglyceride metabolism, fatty acid elongation, fat digestion and absorption, glycerol phospholipid metabolism, steroid The biosynthesis of hormones and the metabolism of inositol phosphate. This shows that there is a significant change in the metabolism of lipids in the fat body of three zolophos treated brown planthopper,.2. interferes with the effect of the acyl coenzyme A oxidase gene on the reproduction of the brown planthopper. The expression of acyl coenzyme A oxidase (Acyl-coenzyme A Oxidase, ACO) in lipid metabolism. In the subsequent RNAi experiment, the reproductive capacity of the brown planthopper containing dsACO artificial diet decreased significantly and.TZP+dsACO treatment decreased by 30% and 32%, respectively, compared with TZP and TZP+dsGFP; the spawning calendar period decreased by 24% and 21%, respectively. The total fat decreased by 20% and 26%. of the female adults of the brown planthopper, and the content of crude fat decreased by 42% (from 5.3mg to 3.1mg) compared with TZP. The content of fatty acids in the body of brown planthopper also changed in varying degrees. The fatty acid, palmitic acid, linoleic acid and stearic acid in the fatty body of the brown planthopper of the TZP+dsACO treated group of brown planthopper. The content of myristic acid, oleic acid, palmitic acid and myristic acid decreased significantly with the ratio of photographing. In addition, the protein content decreased by 11.8% significantly compared with TZP (from 197ug/ five females to five 174ug/ females), indicating that ACO was one of the genes induced by TZP to stimulate BPH reproduction.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S435.112.3

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