本文選題：三七Y病毒 + ci基因　； 參考：《云南農(nóng)業(yè)大學學報(自然科學)》2016年01期

【摘要】：對云南文山地區(qū)三七上獲得的三七Y病毒(Panax virus Y,Pn VY)不同分離物進行了部分ci基因和cp基因的克隆及序列分析,獲得的部分ci基因長657 nt,編碼219個氨基酸;獲得的部分cp基因長834 nt,編碼278個氨基酸。所獲得的11個Pn VY分離物的ci基因之間核苷酸和氨基酸的同源性分別為80.8%~99.8%和91.3%~100%,與其他Pn VY分離物的同源性分別為80.8%~98.8%和92.7%~99.5%。所獲得的18個Pn VY分離物的cp基因核苷酸和氨基酸序列同源性分別為91.8%~99.9%和93.9%~100%,與其他Pn VY分離物的同源性分別為92.0%~100%和95.7%~100%。系統(tǒng)進化樹分析表明,基于ci基因可以將Pn VY分為2個大組,第Ⅱ組分離物之間的差異相比第Ⅰ組的復雜;基于cp基因可以將Pn VY分為3個大組,第Ⅲ組分離物之間差異最大。基于ci基因和cp基因的分析結果均表明,Pn VY不同種群間存在較為豐富的遺傳多樣性,但尚不清楚Pn VY的分子變異是否與Pn VY引起的多種癥狀類型有關。
[Abstract]:The partial ci gene and CP gene were cloned and sequenced from different isolates of Panax virus Y (PN VY) from Panax virus in Wenshan, Yunnan Province. The obtained partial ci gene was 657nt in length and encoded 219 amino acids. Part of the cp gene was 834 NT long, encoding 278 amino acids. The nucleotide and amino acid homology of ci gene of 11 PN VY isolates were 80.8% and 91.3%, respectively. The homology with other PN VY isolates was 80.898% and 92.7% 99.5, respectively. The nucleotide and amino acid sequence homology of CP gene in 18 PN VY isolates were 91.8% and 93.9100%, respectively, and 92.0100% and 95.7% with other PN VY isolates, respectively. Phylogenetic tree analysis showed that PN VY could be divided into two groups based on ci gene, and the difference between group 鈪，

本文編號：2060804