本文選題：miRNA- + Lmo基因�。� 參考：《中國腫瘤生物治療雜志》2017年09期

【摘要】：目的:探討mi R-223調(diào)控急性淋巴細(xì)胞白血病(acute lymphoblastic leukemia,ALL)E6-1細(xì)胞的增殖和體內(nèi)成瘤能力及其作用機(jī)制。方法:應(yīng)用實(shí)時熒光定量PCR檢測mi R-223在不同ALL細(xì)胞株中的表達(dá)水平,免疫熒光染色法檢測攜帶mi R-223mimic慢病毒感染E6-1細(xì)胞株的感染效率,雙熒光素酶實(shí)驗(yàn)檢測mi R-223和Lmo2的相互結(jié)合關(guān)系,MTT增殖實(shí)驗(yàn)和克隆形成實(shí)驗(yàn)檢測過表達(dá)mi R-223對E6-1細(xì)胞株增殖和克隆形成能力的影響。裸鼠體內(nèi)成瘤實(shí)驗(yàn)檢測mi R-223過表達(dá)對E6-1細(xì)胞成瘤能力的影響。Western blotting檢測mi R-223對MAPK信號通路相關(guān)蛋白表達(dá)的影響。結(jié)果:mi R-223在E6-1細(xì)胞株中表達(dá)水平相對較低(P0.05),雙熒光素酶實(shí)驗(yàn)證實(shí)mi R-223可以直接靶向結(jié)合Lmo2的3’UTR區(qū)序列。過表達(dá)mi R-223后:(1)抑制E6-1細(xì)胞的增殖能力(0.16±0.02 vs 1.15±0.21,P0.05);(2)抑制E6-1細(xì)胞的裸鼠體內(nèi)成瘤能力[(0.56±0.08)vs(1.69±0.22)g,P0.05];(3)調(diào)控MAPK信號通路的活性。結(jié)論:mi R-223可靶向作用Lmo2通過MAPK信號通路調(diào)控ALL細(xì)胞的增殖、克隆形成和體內(nèi)成瘤能力。
[Abstract]:Aim: to investigate the effect of miR-223 on the proliferation and tumorigenesis of (acute lymphoblastic leukemiaall (ALL) E6-1 cells. Methods: the expression of miR-223 in different all cell lines was detected by real-time fluorescent quantitative PCR, and the infection efficiency of E6-1 cell line infected with mi R-223 mimic lentivirus was detected by immunofluorescence staining. The relationship between miR-223 and Lmo2 was detected by double luciferase assay. MTT proliferation assay and clone formation assay were used to detect the effect of overexpression of miR-223 on the proliferation and clone formation of E6-1 cell line. The effect of overexpression of mi R-223 on the tumorigenic ability of E6-1 cells was detected by tumorigenesis assay in nude mice. Western blotting was used to detect the effect of mi R-223 on the expression of MAPK-related proteins. Results the expression level of 1: mi R-223 was relatively low in E6-1 cell line (P0.05). The results of double luciferase assay confirmed that miR-223 could directly target the 3 UTR region of Lmo2. After overexpression of miR-223: (1) inhibition of proliferation of E6-1 cells (0.16 鹵0.02 vs 1.15 鹵0.21); (_ 2); inhibition of tumorigenic ability of E6-1 cells in nude mice [(0.56 鹵0.08) vs (1.69 鹵0.22) g P 0.05]; (3) regulation of the activity of MAPKs. Conclusion: 1 / mi R-223 can target Lmo2 to regulate the proliferation, clone formation and tumorigenesis of all cells through MAPK signaling pathway.
【作者單位】： 河南大學(xué)淮河醫(yī)院血液科;
【分類號】：R733.71

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