本文選題：茶樹 + TS��； 參考：《茶葉科學(xué)》2017年03期

【摘要】：茶氨酸合成酶(Theanine synthetase,TS)基因是茶樹茶氨酸代謝過程中的關(guān)鍵酶基因。本研究以氨基酸含量差異明顯的親本及其雜交所得F_1子代為研究材料,克隆TS基因的c DNA序列,挖掘其SNPs位點(diǎn),并成功將雜合SNP位點(diǎn)定位在遺傳連鎖群上。研究結(jié)果顯示,通過序列比對在親本間檢測到3個SNPs,驗(yàn)證得到1個雜合的位點(diǎn)SNP735。將此位點(diǎn)成功轉(zhuǎn)化為dCAPS標(biāo)記,該標(biāo)記在子代中的基因型分離比為1∶1,利用該群體已構(gòu)建的茶樹遺傳圖譜進(jìn)行遺傳定位,將dCAPS標(biāo)記定位在連鎖群LG03上,相鄰標(biāo)記為TM299和TM517。聯(lián)合此標(biāo)記及其相鄰標(biāo)記與游離氨基酸總含量和茶氨酸含量進(jìn)行統(tǒng)計(jì)分析,表明具有顯著的相關(guān)性。
[Abstract]:Theanine synthetase (Theanine synthetase) gene is a key enzyme gene in theanine metabolism of tea plants. In this study, the parents with obvious difference in amino acid content and their F _ 1 progenies were used as the materials to clone the cDNA sequence of TS gene, to excavate its SNPs locus, and to successfully locate the heterozygous SNP loci in the genetic linkage population. The results showed that three SNPs were detected between parents by sequence alignment, and a heterozygous locus SNP735 was identified. The locus was successfully transformed into a dCAPS marker with a genotypic segregation ratio of 1: 1 in the progeny. Using the constructed genetic map of tea tree in this population, the dCAPS marker was mapped to the linkage group LG03, and the adjacent markers were TM299 and TM517. Combined with this marker and its adjacent markers, the total content of free amino acids and theanine content were statistically analyzed.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院茶葉研究所國家茶樹改良中心;
【基金】：國家茶葉產(chǎn)業(yè)技術(shù)體系項(xiàng)目(CARS-023) 國家自然科學(xué)基金(31170624、31500568)
【分類號】：S571.1

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