本文選題：轉(zhuǎn)基因油菜 + 數(shù)字PCR��； 參考：《浙江農(nóng)業(yè)學(xué)報(bào)》2017年05期

【摘要】：隨著轉(zhuǎn)基因產(chǎn)品的大規(guī)模商業(yè)化和我國(guó)大規(guī)模進(jìn)口油菜籽,有效管控存在的風(fēng)險(xiǎn)顯得非常必要和有意義,試驗(yàn)研究了質(zhì)粒標(biāo)準(zhǔn)分子pMs8的研制過(guò)程,包括質(zhì)粒分子的構(gòu)建、純度分析、特異性檢測(cè)、均勻性檢驗(yàn)、穩(wěn)定性考察、定值。結(jié)果表明,該質(zhì)粒分子標(biāo)準(zhǔn)物質(zhì)的內(nèi)源基因和外源基因的均勻性良好,沒(méi)有擴(kuò)增出所選的其他特異性基因,短期穩(wěn)定性在14 d(處理溫度大于25℃)發(fā)生顯著變化,長(zhǎng)期穩(wěn)定性大于6個(gè)月,兩種方法對(duì)外源基因拷貝數(shù)的定值結(jié)果相近,因此,p Ms8是有準(zhǔn)確量值的質(zhì)粒標(biāo)準(zhǔn)分子�？商娲匝芯拷Y(jié)果表明,其可替代基因組作為轉(zhuǎn)基因油菜Ms8定量的陽(yáng)性標(biāo)準(zhǔn)。
[Abstract]:With the large-scale commercialization of transgenic products and the large-scale import of rapeseed in China, it is necessary and meaningful to effectively control the risks. The development process of plasmid standard molecule pMs8, including the construction of plasmid molecule, is studied experimentally. Purity analysis, specificity test, homogeneity test, stability test, fixed value. The results showed that the homogeneity of endogenous gene and exogenous gene of the molecular standard material of the plasmid was good, no other specific genes were amplified, and the short-term stability changed significantly at 14 days (treatment temperature > 25 鈩，

本文編號(hào)：2037233