本文選題：生物信息學 + 甘藍型油菜�。� 參考：《植物學報》2016年05期

【摘要】：在高等植物花藥發(fā)育和花粉形成中,MYB轉(zhuǎn)錄因子起著非常重要的作用,其中MYB80是參與絨氈層發(fā)育及引起雄性不育的重要轉(zhuǎn)錄因子。該研究以擬南芥(Arabidopsis thaliana)At MYB80為參考序列,通過BLAST比對分析,在白菜(Brassica rapa)、甘藍(B.oleracea)和甘藍型油菜(B.napus)中分別獲得MYB80基因的2、2和6個同源序列,運用生物信息學方法對其核苷酸序列及編碼的氨基酸序列進行組成成分、亞細胞定位、磷酸化位點、疏水性/親水性、蛋白質(zhì)二級、三級結(jié)構(gòu)和功能域分析。結(jié)果表明,MYB80轉(zhuǎn)錄因子亞細胞定位于細胞核,具有多個不同的磷酸化位點,肽鏈表現(xiàn)為親水性;二級、三級結(jié)構(gòu)預測顯示,MYB80蛋白以α-螺旋和無規(guī)則卷曲為主要結(jié)構(gòu)元件;保守結(jié)構(gòu)域分析表明,其N端具有2個串聯(lián)的SANT功能域,屬于R2R3型MYB轉(zhuǎn)錄因子。多重序列比對和進化樹分析結(jié)果表明,甘藍型油菜與白菜、甘藍的序列相似性大于92%,且MYB80轉(zhuǎn)錄因子的功能結(jié)構(gòu)域具有較高的同源性和較強的序列保守性。該研究結(jié)果對深入解析甘藍型油菜MYB80的生物學功能及育性調(diào)控的分子機理具有重要意義,為甘藍型油菜雜種優(yōu)勢利用提供了依據(jù)。
[Abstract]:MYB transcription factors play a very important role in anther development and pollen formation of higher plants, among which MYB80 is an important transcription factor involved in tapetum development and male sterility. Using Arabidopsis thaliana)At MYB80 as the reference sequence and blast comparison analysis, two and six homologous sequences of MYB80 gene were obtained in Brassica rapaena, B. oleracea and Brassica napus, respectively. The composition, subcellular localization, phosphorylation site, hydrophobic / hydrophilic, protein secondary, tertiary structure and functional domain were analyzed by bioinformatics. The results showed that the MYB80 transcription factor subcells were located in the nucleus and had many different phosphorylation sites, and the peptide chain was hydrophilic, the second and third order structure prediction showed that the main structural elements of MYB80 protein were 偽 -helix and irregular curl. The analysis of conserved domain showed that the N-terminal had two sant domains, which belonged to R2R3 MYB transcription factor. The results of multiple sequence alignment and phylogenetic tree analysis showed that the sequence similarity between Brassica napus and Brassica campestris was higher than that in Brassica napus, and the functional domain of MYB80 transcription factor had high homology and strong conserved sequence. The results of this study are of great significance in understanding the biological function of MYB80 and the molecular mechanism of fertility regulation in Brassica napus, and provide a basis for the utilization of heterosis in Brassica napus L.
【作者單位】： 西南大學農(nóng)學與生物科技學院;
【基金】：國家重點研發(fā)計劃“七大農(nóng)作物育種”專項(No.2016YFD0101300) 國家自然科學基金(No.U1302266)
【分類號】：S565.4
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本文編號：2009772