本文選題：松材線蟲(chóng) + 蒎烯代謝　； 參考：《林業(yè)科學(xué)》2017年06期

【摘要】：【目的】通過(guò)比較松材線蟲(chóng)CYP450基因和寄主萜烯類物質(zhì)代謝的時(shí)間特異性,研究松材線蟲(chóng)解毒相關(guān)CYP450基因與寄主蒎烯類物質(zhì)代謝之間的相關(guān)性,為該病致病機(jī)制的深入研究提供基礎(chǔ)�！痉椒ā克刹木�蟲(chóng)接種5年生馬尾松后,通過(guò)實(shí)時(shí)定量PCR研究松材線蟲(chóng)CYP450基因和寄主萜烯合成酶基因表達(dá)模式,同時(shí)通過(guò)氣相色譜法檢測(cè)寄主松樹(shù)主要萜烯類物質(zhì)代謝規(guī)律�！窘Y(jié)果】松材線蟲(chóng)接種5年生馬尾松后,松樹(shù)蒎烯合成酶基因分別在第6天和第21天上調(diào)表達(dá),而寄主松樹(shù)體內(nèi)α-蒎烯和β-蒎烯也具有2個(gè)峰值,分別在第9天和第27天時(shí)含量最高。松材線蟲(chóng)CYP-33C9基因在第12天和第15天大量表達(dá),CYP-33C4基因在接種后第21天表達(dá)量最高�！窘Y(jié)論】松材線蟲(chóng)的入侵引起松樹(shù)蒎烯合成酶基因上調(diào)表達(dá),生物合成大量蒎烯類物質(zhì),松材線蟲(chóng)CYP450基因響應(yīng)松樹(shù)蒎烯類物質(zhì)的大量積累而上調(diào)表達(dá)。因此,根據(jù)松材線蟲(chóng)CYP450基因與松樹(shù)蒎烯類物質(zhì)代謝在時(shí)間上的相關(guān)性,推測(cè)CYP450基因可能參與了松材線蟲(chóng)蒎烯類物質(zhì)代謝過(guò)程,可能是松材線蟲(chóng)致病相關(guān)基因之一。
[Abstract]:[objective] to study the correlation between CYP450 gene of pine wood nematode and the metabolism of host pinene by comparing the time specificity of CYP450 gene with that of host terpene. [methods] the expression patterns of CYP450 gene and host terpene synthase gene of pine wood nematode were studied by real-time quantitative PCR after inoculated with 5-year-old Pinus massoniana. At the same time, the metabolism of main terpenes in host pine trees was detected by gas chromatography. [results] after pine wood nematodes were inoculated with 5-year-old Pinus massoniana, the expression of pinene synthase gene was up-regulated on the 6th and 21st day, respectively. There were two peak values of 偽 -pinene and 尾 -pinene in host pine, which were the highest on the 9th and 27th day, respectively. CYP-33C9 gene of pine wood nematode expressed in large quantities on the 12th and 15th day after inoculation. [conclusion] the invasion of pine wood nematode caused the up-regulation of pinene synthase gene and biosynthesis of a large number of pinene compounds. CYP450 gene of pine wood nematode was up-regulated in response to the accumulation of pinene. Therefore, according to the correlation between CYP450 gene of pine wood nematode and the metabolism of pinene in pine tree, it is speculated that CYP450 gene may be involved in the metabolism of pinene of pine wood nematode, and it may be one of the genes related to the pathogenicity of pine wood nematode.
【作者單位】： 中國(guó)林業(yè)科學(xué)研究院林業(yè)新技術(shù)研究所;中國(guó)林業(yè)科學(xué)研究院森林生態(tài)環(huán)境與保護(hù)研究所國(guó)家林業(yè)局森林保護(hù)學(xué)重點(diǎn)實(shí)驗(yàn)室;山東農(nóng)業(yè)大學(xué);
【基金】：浙江省省院合作林業(yè)科技項(xiàng)目(2014SY17) 林業(yè)公益性行業(yè)科研專項(xiàng)經(jīng)費(fèi)(201204501)
【分類號(hào)】：S763.18

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1 王璇;理永霞;劉振宇;呂全;賈秀貞;張星耀;;松材線蟲(chóng)CYP450基因與松樹(shù)蒎烯類物質(zhì)代謝的相關(guān)性[J];林業(yè)科學(xué);2017年06期

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本文編號(hào)：1981503