本文選題：海南 + 五指山豬　； 參考：《黑龍江畜牧獸醫(yī)》2017年17期

【摘要】：為了探討海南五指山豬TLR2基因的分子結(jié)構(gòu)特征及相關(guān)遺傳變異,為研究TLR2基因遺傳變異與免疫疾病的關(guān)系打下基礎(chǔ),試驗(yàn)采用PCR技術(shù)對(duì)該基因組序列進(jìn)行克隆、測(cè)序及相關(guān)生物信息學(xué)分析。結(jié)果表明:獲得2 649 bp的五指山豬TLR2基因的DNA序列,其基因編碼區(qū)全長(zhǎng)2 358 bp,編碼785個(gè)氨基酸;與Gen Bank中發(fā)布的普通豬TLR2基因序列相比,共發(fā)現(xiàn)9處突變,其中在編碼區(qū)序列存在2處突變,屬于有義突變;與普通豬、馬、牛、綿羊、人、黑猩猩、獼猴、犬、小鼠、雞、斑馬魚(yú)的氨基酸序列的一致性分別為99.7%、82.1%、81.4%、80.6%、78.4%、78.4%、77.8%、77.3%、68.2%、50.7%、40.4%;五指山豬TLR2編碼的蛋白主要位于質(zhì)膜,屬于分泌性蛋白、親水性蛋白和跨膜蛋白,有信號(hào)肽和5個(gè)糖基化修飾位點(diǎn),存在較多的絲氨酸(Ser)和蘇氨酸(Thr)磷酸化位點(diǎn);不同物種TLR2基因在進(jìn)化過(guò)程中具有較高的保守性。說(shuō)明TLR2基因可作為今后研究海南五指山豬免疫疾病的重要指標(biāo)之一。
[Abstract]:In order to study the molecular structure and related genetic variation of TLR2 gene in Wuzhishan pig, Hainan, and to study the relationship between genetic variation of TLR2 gene and immune disease, the genomic sequence was cloned by PCR technique. Sequencing and bioinformatics analysis. The results showed that the DNA sequence of the TLR2 gene of Wuzhishan pig was 2 649 BP, and its coding region was 2 358 BP, encoding 785 amino acids, and 9 mutations were found compared with the normal TLR2 gene sequence published in Gen Bank. There are 2 mutations in the coding region, which belong to the sense mutation; and ordinary pigs, horses, cattle, sheep, human beings, chimpanzees, macaques, dogs, mice, chickens, The amino acid sequences of zebrafish were 99.72.1and 81.4, respectively. The amino acid sequences of zebrafish were 78.4 and 78.4, respectively. The protein encoded by Wuzhishan pig TLR2 was mainly located in the plasma membrane, which belonged to secreted protein, hydrophilic protein and transmembrane protein, and had signal peptides and five glycosylation modification sites. There were more serine and threonine Thr-phosphorylation sites, and TLR2 genes of different species were highly conserved in the course of evolution. The results suggest that TLR2 gene can be used as one of the important indexes for the future study of immune diseases in Wuzhishan pigs in Hainan.
【作者單位】： 海南省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所;海南省熱帶動(dòng)物繁育與疫病研究重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31560696) 海南省自然科學(xué)基金項(xiàng)目(20153080) 海南省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)科技創(chuàng)新專項(xiàng)(CXZX201504)
【分類號(hào)】：Q78;S828

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 馮書(shū)堂,陳幼春;五指山豬生物學(xué)特性、易地繁育及遺傳多樣性研究[J];生物多樣性;1998年03期

2 侯冠_g;曾鴻普;周漢林;王東勁;管松;馬月輝;;五指山豬GH、IGFBP3基因SNPs檢測(cè)及與生長(zhǎng)性狀的關(guān)聯(lián)分析[J];基因組學(xué)與應(yīng)用生物學(xué);2010年03期

相關(guān)會(huì)議論文 前1條

1 常懷普;歐江濤;鐘金城;王希龍;郭春華;賓石玉;馬志杰;;五指山豬IGF2基因5'調(diào)控區(qū)的單核苷酸多態(tài)性分析[A];中國(guó)遺傳學(xué)會(huì)功能基因組學(xué)研討會(huì)論文集[C];2006年

相關(guān)碩士學(xué)位論文 前1條

1 王曉東;五指山豬SLA-DQA基因的克隆及在大腸桿菌的表達(dá)[D];甘肅農(nóng)業(yè)大學(xué);2006年

，

本文編號(hào)：1971087