發(fā)布時(shí)間：2018-06-02 04:49

  本文選題：玉米 + 氨基酸通透酶(AAP)��； 參考：《玉米科學(xué)》2017年01期

【摘要】：氨基酸通透酶1(AAP1)是位于細(xì)胞膜上與氨基酸轉(zhuǎn)運(yùn)有關(guān)的載體蛋白。以轉(zhuǎn)組成型啟動(dòng)子(UBI)的AAP1玉米植株(N7-30、N7-26)、轉(zhuǎn)胚乳特異性啟動(dòng)子(Glu)的AAP1玉米植株(KF8-33、KF8-39、KF9-22)為材料,探究轉(zhuǎn)AAP1基因玉米中游離氨基酸和可溶性蛋白質(zhì)含量之間的變化關(guān)系。結(jié)果表明,轉(zhuǎn)AAP1基因玉米的根部和葉片中游離氨基酸含量在抽絲期最高;根部可溶性蛋白質(zhì)含量在成熟期最高;葉片中可溶性蛋白質(zhì)含量在抽絲期最高。葉片中游離氨基酸含量與可溶性蛋白質(zhì)含量隨著生育期的變化均呈現(xiàn)低-高-低的趨勢(shì),抽絲期達(dá)到最高值;種子中游離氨基酸的含量與可溶性蛋白質(zhì)含量呈顯著正相關(guān)性。轉(zhuǎn)胚乳特異性啟動(dòng)子玉米種子中游離氨基酸含量、脯氨酸含量、賴氨酸含量均顯著高于轉(zhuǎn)組成型啟動(dòng)子玉米種子。AAP1可以提高植物對(duì)游離氨基酸的吸收與轉(zhuǎn)運(yùn),進(jìn)而提高植物對(duì)氮素的利用效率。
[Abstract]:Amino acid permease 1 (AAP1) is a carrier protein related to amino acid transport on the cell membrane. The relationship between the content of free amino acids and soluble protein in transgenic AAP1 maize was studied by using AAP1 maize plant (KF8-33KF8-39KF9-22), which was transformed into AAP1 maize plant N7-30 (N7-26) and transferred endosperm specific promoter (Glu.). The results showed that the content of free amino acids in roots and leaves of transgenic AAP1 maize was the highest at the stage of silk drawing, the content of soluble protein in roots was the highest in mature stage, and the content of soluble protein in leaves was the highest at the stage of silk drawing. The content of free amino acid and soluble protein in leaves showed a low-high-low trend with the change of growth stage, and reached the highest value at the stage of silk drawing, and the content of free amino acid in seeds was significantly positively correlated with the content of soluble protein. The content of free amino acid, proline and lysine in transgenic corn seeds were significantly higher than those in transgenic promoter maize seeds. AAP1 could improve the absorption and transport of free amino acids. Therefore, the utilization efficiency of nitrogen in plants was improved.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【基金】：吉林省科技廳重點(diǎn)科技攻關(guān)項(xiàng)目(20130206012NY) 吉林省應(yīng)用基礎(chǔ)研究重點(diǎn)實(shí)驗(yàn)室開(kāi)放課題項(xiàng)目(20130102066JC)
【分類號(hào)】：S513
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本文編號(hào)：1967484