拉薩白雞群體魚腥味敏感基因多態(tài)性分析
發(fā)布時(shí)間:2018-05-31 07:44
本文選題:拉薩白雞 + 魚腥味; 參考:《中國家禽》2017年22期
【摘要】:雞含黃素單氧化酶(FMO3)基因突變可導(dǎo)致雞蛋出現(xiàn)魚腥味,因此稱為魚腥味基因。研究旨在采用PCR-RFLP方法檢測(cè)63個(gè)拉薩白雞個(gè)體中FMO3基因T329S突變位點(diǎn)的基因及基因型頻率分布,為提高該群體的蛋品質(zhì)提供數(shù)據(jù)。結(jié)果顯示:拉薩白雞群體中,AA、AT和TT(魚腥味綜合癥易感基因型)基因型頻率分別為90.48%、9.52%和0,A和T的基因頻率分別為95.24%、4.76%。該位點(diǎn)基因型分布處于Hardy-Weinberg平衡狀態(tài)。拉薩白雞群體中T等位基因的頻率為4.76%,雖然魚腥味綜合征易感個(gè)體(TT基因型)期望頻率很低,但應(yīng)該通過分子輔助選擇技術(shù)予以剔除。
[Abstract]:Chicken FMO3) gene mutation can lead to fishy smell in eggs, so it is called fishy smell gene. The aim of this study was to detect the gene and genotype frequency distribution of T329S mutation of FMO3 gene in 63 Lhasa white chickens by using PCR-RFLP method, and to provide data for improving egg quality of this population. The results showed that the genotypic frequencies of AA AT and TT (susceptible genotype of fishy smell syndrome) in Lhasa white chicken population were 90.48% and 9.52%, respectively, and the frequencies of 0A and T were 95.2424 and 4.76, respectively. The genotype distribution of this locus is in Hardy-Weinberg equilibrium state. The frequency of T allele in Lhasa white chicken population was 4.76. Although the expected frequency of T allele was very low in the susceptible individuals with fishy smell syndrome, it should be eliminated by molecular assisted selection.
【作者單位】: 中國農(nóng)業(yè)大學(xué)動(dòng)物遺傳資源與分子育種實(shí)驗(yàn)室;西藏自治區(qū)農(nóng)牧科學(xué)院畜牧獸醫(yī)研究所;
【基金】:現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金(CARS-41)
【分類號(hào)】:S831
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本文編號(hào):1958847
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