本文選題：子癇前期 + PKIP基因��； 參考：《實用婦產(chǎn)科雜志》2017年08期

【摘要】：目的:探究P57KIP2基因過表達(dá)之后對滋養(yǎng)層細(xì)胞生物學(xué)行為的影響。方法:采用實時熒光定量(q PCR)及Western Blot方法檢測人絨毛膜滋養(yǎng)層細(xì)胞(HTR8-/SVneo)過表達(dá)P57KIP2基因后mRNA和蛋白水平的表達(dá)變化;用Transwell方法檢測HTR8-/SVneo細(xì)胞過表達(dá)P57KIP2基因后遷移、侵襲能力生物學(xué)行為的變化;CCK8檢測HTR8-/SVneo細(xì)胞過表達(dá)該基因后增殖能力的變化。采用免疫熒光檢測HTR8-/SVneo細(xì)胞過表達(dá)該基因后,抗凋亡蛋白Bcl2的表達(dá)變化。結(jié)果:滋養(yǎng)層細(xì)胞過表達(dá)P57KIP2基因后,其mRNA及蛋白水平均明顯增高(P0.05);P57KIP2基因過表達(dá)后滋養(yǎng)層細(xì)胞的遷移、侵襲數(shù)量均明顯增高,差異有統(tǒng)計學(xué)意義(P0.05);滋養(yǎng)層細(xì)胞的細(xì)胞相對吸光值48小時后明顯增高(P0.001);過表達(dá)P57KIP2基因后滋養(yǎng)層細(xì)胞中抗凋亡蛋白Bcl2表達(dá)增高(P0.05)。結(jié)論:P57KIP2基因可能會增加滋養(yǎng)層細(xì)胞遷移、侵襲以及增殖能力,P57KIP2基因可能參與調(diào)控滋養(yǎng)細(xì)胞的凋亡過程。
[Abstract]:Objective: to investigate the effect of overexpression of P57KIP2 gene on the biological behavior of trophoblast cells. Methods: the expression of mRNA and protein in human chorionic trophoblastic cells (HTR8-SVneoa) was detected by real-time fluorescence quantitative PCR and Western Blot methods, and the expression of mRNA and protein in HTR8-/SVneo cells after overexpression of P57KIP2 gene was detected by Transwell method. The change of biological behavior of invasion ability and the change of proliferative ability of HTR8-/SVneo cells after overexpression of the gene were detected by CCK8. The expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence after overexpression of the gene in HTR8-/SVneo cells. Results: after overexpression of P57KIP2 gene in trophoblast cells, the levels of mRNA and protein in trophoblast cells were significantly increased, and the migration and invasion of trophoblast cells were significantly increased after overexpression of P0.05 and P57KIP2 gene. The difference was statistically significant (P 0.05), the relative absorptivity of trophoblast cells was significantly increased after 48 hours, and the expression of anti-apoptotic protein (Bcl2) in trophoblast cells was increased after overexpression of P57KIP2 gene. Conclusion the P57KIP2 gene may increase the migration, invasion and proliferation of trophoblast cells. P57KIP2 gene may be involved in the regulation of trophoblast apoptosis.
【作者單位】： 四川大學(xué)華西第二醫(yī)院出生缺陷與相關(guān)婦兒疾病教育部重點實驗室;
【基金】：國家自然科學(xué)基金項目(編號:81471463) 四川省科技廳課題(編號:2014JY0158)
【分類號】：R714.244

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