本文選題：禾谷縊管蚜 + Hsp　； 參考：《植物保護(hù)學(xué)報》2017年01期

【摘要】：為探討高溫馴化對禾谷縊管蚜Rhopalosiphum padi Hsp90基因表達(dá)量的影響,以及其在不同發(fā)育階段的表達(dá),通過RT-PCR與RACE技術(shù)克隆了熱激蛋白Hsp90基因的全長,命名為Rp Hsp90,采用生物信息方法分析了其序列特征,利用實(shí)時熒光定量PCR技術(shù)研究了Rp Hsp90在禾谷縊管蚜不同發(fā)育階段和馴化后熱激處理的表達(dá)量變化。結(jié)果顯示:禾谷縊管蚜Rp Hsp90的c DNA全長為2 644 bp,編碼727個氨基酸,分子量為83.2 k D。推導(dǎo)的氨基酸序列具有Hsp90蛋白家族的5個簽名序列及C末端細(xì)胞質(zhì)特征序列EEVD。系統(tǒng)進(jìn)化樹結(jié)果顯示,Rp Hsp90與其它昆蟲Hsp90具有很高的相似性。實(shí)時熒光定量PCR結(jié)果表明,不同發(fā)育階段Rp Hsp90均有表達(dá),并且4齡若蚜期表達(dá)量最高,顯著高于其它齡期;27℃熱馴化后禾谷縊管蚜Rp Hsp90表達(dá)量和常溫24℃處理試蟲無顯著差異;熱激處理顯著誘導(dǎo)Rp Hsp90的表達(dá),39℃熱激處理Rp Hsp90表達(dá)量顯著高于40℃熱激處理;熱馴化種群經(jīng)熱激處理后表達(dá)量顯著高于常溫?zé)峒ぬ幚�。表明Rp Hsp90在禾谷縊管蚜不同齡期差異表達(dá),且在該蟲對熱脅迫的響應(yīng)中具有重要作用。
[Abstract]:In order to investigate the effect of high temperature acclimatization on the expression of Rhopalosiphum PADI Hsp90 gene of cereal constrictive aphid and its expression at different developmental stages, the whole length of the gene of heat shock protein Hsp90 was cloned by RT-PCR and RACE technology, named Rp Hsp90, and its sequence characteristics were analyzed by biological information method, and the real-time fluorescent quantitative PCR technology was used. The changes in the expression of Rp Hsp90 at different stages of development and heat shock after acclimation were investigated. The results showed that the total length of C DNA of the cereal constriction aphid Rp Hsp90 was 2644 BP, 727 amino acids encoded and the molecular weight of 83.2 K D. had 5 signature sequences of the Hsp90 protein family and the C terminal cytoplasmic sequence EEVD. The phylogenetic tree showed that Rp Hsp90 had high similarity with other insect Hsp90. Real time fluorescence quantitative PCR showed that Rp Hsp90 was expressed in different developmental stages, and the expression of aphid stage at 4 age was the highest and significantly higher than that of other ages. The expression of Rp Hsp90 in cereal constrictive aphid and at 24 C at normal temperature were not significant after thermal acclimation at 27. The expression of Rp Hsp90 was significantly induced by heat shock treatment, and the expression of Rp Hsp90 in heat shock treatment at 39 C was significantly higher than that of heat shock treatment at 40 C. The expression of heat acclimated population after heat shock treatment was significantly higher than that at normal temperature. It showed that Rp Hsp90 was expressed in different ages of cereal constriction aphid, and it was important in the response of the insect to heat stress.
【作者單位】： 西北農(nóng)林科技大學(xué)植物保護(hù)學(xué)院農(nóng)業(yè)部西北黃土高原作物有害生物綜合治理重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(31272036,31471766) 教育部高等學(xué)校博士學(xué)科點(diǎn)專項科研基金(20110204110001)
【分類號】：S433

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