本文選題：擬南芥 + 突變體庫　； 參考：《氨基酸和生物資源》2016年04期

【摘要】：我國油菜含油量低,嚴(yán)重影響了國產(chǎn)菜籽的市場競爭力,提高含油量是油菜育種的當(dāng)務(wù)之急。擬南芥與油菜同屬十字花科,利用擬南芥篩選油脂合成及調(diào)控相關(guān)的新基因并將其應(yīng)用于油菜,是快速選育高油油菜品種的有效手段。本研究采用密度梯度離心方法及核磁共振檢測油菜種子的含油量,從6 868份擬南芥突變體庫資源中篩選得到9個種子含油量較野生型呈顯著性差異的株系。通過T-DNA插入突變的特點,篩選得到6個調(diào)控種子油脂合成的候選基因:AT2G19450、AT3G26790、AT3G54320、AT5G06270、AT2G21840和AT3G47450,其中AT5G06270,AT2G21840和AT3G47450屬于未知功能基因,為深入研究種子含油量和其調(diào)控機(jī)理提供了一定的參考。
[Abstract]:The low oil content of rapeseed in China has seriously affected the market competitiveness of domestic rapeseed. It is urgent to improve the oil content in rapeseed breeding. Arabidopsis thaliana and Brassica napus belong to the cruciferous family. It is an effective method to select new genes related to oil synthesis and regulation and to apply them to rapeseed by using Arabidopsis thaliana (Arabidopsis thaliana) and Brassica napus. In this study, the oil content of rape seed was detected by density gradient centrifugation and nuclear magnetic resonance (NMR). Nine lines with significant difference in oil content were obtained from 6868 Arabidopsis mutants. Six candidate genes AT2G19450 AT3G26790 AT3G54320 (AT5G06270) AT2G21840 and AT3G47450 (AT5G06270) and AT3G47450 (AT5G06270 / AT2G21840 and AT3G47450), which are unknown functional genes, were selected to control seed oil synthesis.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院油料作物研究所/農(nóng)業(yè)部油料作物生物學(xué)與遺傳育種重點實驗室;常德市農(nóng)林科學(xué)研究院油料研究所;
【基金】：國家863計劃重點項目(2013AA102602)
【分類號】：Q943.2

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