本文選題：棉花 + GhbHLH��； 參考：《分子植物育種》2017年04期

【摘要】：bHLH(basic helix-loop-helix,bHLH)家族基因在植物生長發(fā)育和非生物脅迫應(yīng)答中發(fā)揮著重要的作用。本研究從前期構(gòu)建的棉花cDNA文庫中發(fā)掘得到一個新的GhbHLH基因,該基因開放閱讀框全長600 bp,編碼199個氨基酸,序列分析表明,該基因具有典型的helix-loop-helix結(jié)構(gòu)域。熒光定量PCR表明,GhbHLH在棉花根、莖、葉和纖維中均有表達,并且能夠受高鹽、PEG、低溫、ABA和Me JA等的誘導(dǎo)表達,亞細胞定位結(jié)果表明,GhbHLH在細胞核內(nèi)表達。推測該基因可能作為調(diào)控因子在棉花非生物脅迫應(yīng)答中發(fā)揮作用。
[Abstract]:The genes of bHLH(basic helix-loop-helixhh family play an important role in plant growth and development and abiotic stress response. In this study, a new GhbHLH gene was obtained from the cotton cDNA library constructed in the previous period. The open reading frame of the gene was 600bp in length, encoding 199 amino acids. Sequence analysis showed that the gene had a typical helix-loop-helix domain. Fluorescence quantitative PCR showed that GhbHLH was expressed in cotton roots, stems, leaves and fibers, and could be induced by PEG, low temperature ABA and me JA. The subcellular localization results showed that GhbHLH was expressed in the nucleus. It is speculated that this gene may play a role as a regulatory factor in cotton abiotic stress response.
【作者單位】： 河北省農(nóng)林科學院棉花研究所農(nóng)業(yè)部黃淮海半干旱區(qū)棉花生物學與遺傳育種重點實驗室;河北行政學院;
【基金】：棉花生物學國家重點實驗室開放課題(CB2015A19) 河北省農(nóng)林科學院基本科研業(yè)務(wù)費(A2015070102) 河北省財政項目(F16R2016024592)共同資助
【分類號】：Q943.2;S562
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本文編號：1889435