本文選題：板栗 + CAT基因��； 參考：《食品工業(yè)科技》2017年13期

【摘要】：為研究板栗過氧化氫酶基因CAT1的功能,旨在為其采后貯藏中品質(zhì)變化研究提供分子機理基礎(chǔ)。以板栗為試材,提取總RNA,通過c DNA末端快速擴增技術(shù)(RACE),克隆到板栗CAT1基因c DNA全長,并對其進行生物信息學(xué)分析。結(jié)果表明,該基因序列全長1485 bp,包含一個1479 bp的開放閱讀框,編碼492個氨基酸。氨基酸序列分析表明,板栗CAT1與棗(Ziziphus jujuba)、煙草(Nicotiana tabacum)、陸地棉(Gossypium hirsutum)等植物過氧化氫酶CAT1氨基酸序列有較高的相似性。蛋白分析表明,該蛋白分子量為56947.31 Da,理論等電點6.65,屬穩(wěn)定性親水蛋白;亞細胞定位于過氧化氫酶體,存在30個磷酸化位點,無信號肽。二級結(jié)構(gòu)以α螺旋、無規(guī)則卷曲為主。保守結(jié)構(gòu)域預(yù)測表明,該基因編碼蛋白屬于Catalase-like超家族。本研究克隆獲得了板栗CAT1基因,為進一步研究該基因的生物學(xué)功能奠定了基礎(chǔ)。
[Abstract]:In order to study the function of catalase gene CAT1 in Chinese chestnut, the purpose of this study was to provide a molecular basis for the study of quality change in postharvest storage. Total RNAs were extracted from Chinese chestnut and cloned into chestnut CAT1 gene c DNA by rapid amplification technique of c DNA terminal, and then analyzed by bioinformatics. The results showed that the gene was 1485 BP in length and contained a 1479 BP open reading frame encoding 492 amino acids. Amino acid sequence analysis showed that the amino acid sequence of catalase CAT1 in Chinese chestnut CAT1 and Ziziphus jujuba, Nicotiana tabacumum and Gossypium hirsutum were similar. Protein analysis showed that the protein had a molecular weight of 56947.31 Da and a theoretical isoelectric point of 6.65, which was a stable hydrophilic protein, and the subcell was located in catalase with 30 phosphorylation sites and no signal peptide. The secondary structure is 偽-helix and irregular crimp. The prediction of conserved domain showed that the gene encoded protein belonged to Catalase-like superfamily. In this study, the CAT1 gene of Chinese chestnut was cloned, which laid a foundation for further study of the biological function of the gene.
【作者單位】： 寧波大學(xué)食品科學(xué)與工程系應(yīng)用海洋生物技術(shù)教育部重點實驗室;
【分類號】：Q943.2;S664.2
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本文編號：1889136