本文選題：惡性瘧原蟲 + 裂殖子表面蛋白　； 參考：《中國寄生蟲學(xué)與寄生蟲病雜志》2017年05期

【摘要】：目的了解武漢市輸入性惡性瘧原蟲(Plasmodium falciparum)裂殖子表面蛋白1(merozoite surface protein 1,MSP1)和MSP2的等位基因型特征。方法于2010-2015年采集武漢市從非洲瘧疾流行區(qū)回國人員中惡性瘧現(xiàn)癥患者血樣,收集患者流行病學(xué)資料。提取患者血樣中的惡性瘧原蟲DNA,采用巢式PCR分別擴(kuò)增惡性瘧原蟲MSP1、MSP2基因型特異性片段,分析各等位基因型的構(gòu)成比和不同感染來源地患者的基因型頻數(shù)分布,分析重癥患者不同基因型的相關(guān)性。不同基因型的重癥患者比例差異采用χ~2檢驗。結(jié)果共采集輸入性惡性瘧患者血樣175份,其中輕度癥狀患者血樣137份,重癥患者血樣38份。巢式PCR結(jié)果顯示,MSP1等位基因K1、RO33、MAD20型分別擴(kuò)增出260~390、270和150 bp目的條帶;MSP2等位基因3D7、FC27型分別擴(kuò)增出200~330、330~500 bp目的條帶。MSP1和MSP2基因均未檢出的有9份。檢出MSP1等位基因136份,檢出率為77.7%,等位基因MAD20、K1、RO33、MAD20+K1、MAD20+RO33、K1+RO33、MAD20+K1+RO33型的構(gòu)成比分別為5.1%(7/136)、37.5%(51/136)、20.6%(28/136)、5.9%(8/136)、4.4%(6/136)、16.9%(23/136)、9.5%(13/136)。檢出MSP2等位基因143份,檢出率為81.7%,FC27、3D7和FC27+3D7型的構(gòu)成比為20.2%(29/143)、39.9%(57/143)、39.9%(57/143)。MAD20型和K1以南非最多,分別占10.5%(4/38)和34.2%(13/38);RO33型以東非最多,占2/7;FC27型東非和北非均未檢出,南非占比最高,為18.4%(7/38);3D7型以東非最多,占4/7。MSP1基因MAD20、K1、RO33、MAD20+K1、MAD20+RO33、K1+RO33、MAD20+K1+RO33型的重癥患者分別占1/7、19.6%(10/51)、32.1%(9/28)、1/8、4/6、13.0%(3/23)、46.2%(6/13),MAD20+RO33、MAD20+K1+RO33和RO33(P0.05),與其他4種基因型間差異有統(tǒng)計學(xué)意義(P0.05)。MSP2基因3D7、FC27、FC27+3D7型的重癥患者比例分別為19.3%(11/57)、20.7%(6/29)、24.6%(14/57),各基因型間差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論武漢市輸入性惡性瘧原蟲MSP1存在MAD20、Kl和R033等3種單基因型以及4種多基因型,以K1型和R033型為優(yōu)勢基因型;MSP2存在FC27、3D7和FC27+3D7型,3D7型的比例大于FC27型。
[Abstract]:Objective to investigate the allelic characteristics of merozoite surface protein (1(merozoite surface protein 1 MSP1) and MSP2 of Plasmodium falciparum in Wuhan. Methods Blood samples of patients with falciparum malaria from malaria endemic areas in Africa were collected from 2010 to 2015 in Wuhan. The plasmodium falciparum DNA was extracted from the blood samples of the patients, and the specific fragments of MSP1mSP2 genotypes were amplified by nested PCR. The composition ratio of each allele and the frequency distribution of the genotypes in patients with different infection sources were analyzed. To analyze the correlation of different genotypes in severe patients. The proportion of severe patients with different genotypes was tested by 蠂 2 test. Results 175 blood samples were collected from imported falciparum malaria patients, including 137 samples from patients with mild symptoms and 38 samples from patients with severe symptoms. The results of nested PCR showed that the MSP1 allele K1 / RO33 / MAD20 was amplified from 260h390270 and 150bp / mSP2 allele 3D7FFC27, respectively, and 9 of them were not detected in the target band. MSP1 and MSP2 genes were not detected. A total of 136 MSP1 alleles were detected, and the detection rate was 77.7.The ratio of MAD20K1 / MAD20K1and MAD20 RO33K1 / MAD20K1 RO33 was 5.1R / 7136C 37.5N = 51R / 7136C 20.6ng / 288136N 5.9 / 1364.4m = 16.6 / 166N = 16.923 / 136P / 169.136. There were 143 MSP2 alleles detected, and the detection rate was 81.7FC273D7 and FC27 3D7, and the ratio was 20.229 / 143D7 and 39.929 / 143Q / 39.90.57 / 143t0 / 39.9nb, the highest number were in South Africa (10.54r.38) and 34.21338P / RO33, respectively, in East Africa, and in 2 / 7FC27 East Africa and North Africa, the highest in South Africa, 18.47% in East Africa, 3D7% in East Africa, and 3D7% in East Africa. 鍗，

本文編號：1890202