本文選題：幽門(mén)螺桿菌 + 胃癌��； 參考：《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2017年18期

【摘要】：目的探討核苷酸結(jié)合寡聚化結(jié)構(gòu)域(NOD)1和2基因在幽門(mén)螺桿菌(Hp)相關(guān)胃癌患者中的表達(dá)變化及意義。方法采用免疫組織化學(xué)法檢測(cè)胃癌患者癌組織中NOD1和NOD2蛋白的表達(dá);采用實(shí)時(shí)聚合酶鏈反應(yīng)(real-time PCR)檢測(cè)胃癌組織中NOD1和NOD2 m RNA的表達(dá);采用Western blot檢測(cè)胃癌組織中凋亡蛋白核轉(zhuǎn)錄因子κB(NF-κB)和半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)表達(dá)。結(jié)果與Hp陰性胃癌患者相比,Hp陽(yáng)性胃癌患者癌組織中的NOD1和NOD2蛋白表達(dá)含量和m RNA表達(dá)含量上升;Hp陽(yáng)性胃癌患者中的NF-κB和Caspase-3蛋白含量升高(P0.05)。結(jié)論 Hp陽(yáng)性胃癌患者中的NOD1和NOD2呈高表達(dá)狀態(tài);且過(guò)表達(dá)NOD1和NOD2可能在Hp陽(yáng)性胃癌發(fā)生、發(fā)展機(jī)制中起重要的作用。
[Abstract]:Objective to investigate the expression and significance of nucleotide-binding oligodeoxynucleotidyl and oligodeoxynucleotides (Oligomeric domain) 1 and 2 genes in patients with Helicobacter pylori (HP)-associated gastric cancer. Methods the expression of NOD1 and NOD2 in gastric cancer was detected by immunohistochemistry, and the expression of NOD1 and NOD2 m RNA was detected by real-time PCR. Western blot was used to detect the expression of apoptotic protein NF- 魏 B (NF- 魏 B) and cysteine (caspase-3) of aspartate proteolytic enzyme (Caspase-3) in gastric carcinoma. Results compared with the patients with HP negative gastric cancer, the expression of NOD1 and NOD2 protein and the expression of m RNA were increased in the patients with HP positive gastric cancer. The content of NF- 魏 B and Caspase-3 protein in the patients with HP positive gastric cancer was higher than that in the patients with HP positive gastric cancer (P 0.05). Conclusion the expression of NOD1 and NOD2 in patients with HP positive gastric cancer is high, and overexpression of NOD1 and NOD2 may play an important role in the pathogenesis of HP positive gastric cancer.
【作者單位】： 河北醫(yī)科大學(xué)口腔學(xué)院;河北醫(yī)科大學(xué)附屬哈勵(lì)遜國(guó)際和平醫(yī)院病理科;
【分類(lèi)號(hào)】：R735.2

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