本文選題：誘導(dǎo)多能干細胞 + 自發(fā)性分化　； 參考：《神經(jīng)解剖學(xué)雜志》2017年03期

【摘要】：目的:研究小鼠誘導(dǎo)多能干細胞(i PS細胞)在體外自發(fā)性分化和神經(jīng)定向分化過程中的基因表達譜的特性。方法:在體外將i PS細胞分別進行形成類胚胎小體后自發(fā)性分化及成骨蛋白抑制劑Noggin誘導(dǎo)神經(jīng)定向分化后,通過實時熒光定量PCR(q PCR)測定在分化過程中i PS細胞分化相關(guān)基因表達的變化。結(jié)果:i PS細胞形成類胚胎小體后胚胎外胚層標(biāo)志基因(GFAP,Map2和Tu J1)及內(nèi)胚層標(biāo)記基因(Foxa2,GATA4和Sox17)的表達隨分化而迅速增加,但中胚層標(biāo)記基因(Bmp4,BRA,FGF5)表達水平變化不明顯。在神經(jīng)定向分化過程中,i PS細胞的神經(jīng)標(biāo)志物基因(Map2,Neu N,Tu J1和Sox1)及線粒體相關(guān)基因(12s,ND3,ND5,ND6、Cytb和Cox1)的表達都逐漸增加,且兩者具有相同的增長趨勢。結(jié)論:在自發(fā)性分化過程中小鼠i PS細胞具有自發(fā)向外胚層和內(nèi)胚層分化的趨勢;在神經(jīng)定向分化過程中,i PS細胞的線粒體相關(guān)基因表達隨著分化表達逐漸增加,并與神經(jīng)細胞標(biāo)記基因具有正相關(guān)性,表明線粒體的功能在i PS細胞神經(jīng)定向分化中發(fā)揮重要作用。
[Abstract]:Aim: to study the characteristics of gene expression profile of mouse induced pluripotent stem cell / PS cells during spontaneous and neuronal differentiation in vitro. Methods: in vitro, I PS cells were divided into embryoid bodies after spontaneous differentiation and osteogenic protein inhibitor Noggin induced neuronal directional differentiation. The expression of differentiation related genes in iPS cells was measured by real-time fluorescence quantitative PCR(q. Results the expression of embryonic ectodermal marker genes (GFAPMap2 and Tu J1) and endodermal marker genes (Foxa2 (GATA4) and Sox17) increased rapidly with differentiation, but the expression level of mesodermal marker gene (Bmp4, BRAFGF5) did not change significantly. During the process of neuronal directional differentiation, the expression of neuronal marker gene (map2Neu Nu Tu J1 and Sox1) and mitochondrial associated gene (12sNd3Nd5Nd5Nd6, Cytb and Cox1) in PS cells increased gradually, and both of them had the same increasing trend. Conclusion: the spontaneous differentiation of mouse iPS cells into ectoderm and endoderm during spontaneous differentiation and the expression of mitochondria related genes in iPS cells increased with the differentiation. There is a positive correlation between mitochondria and neuronal marker gene, which indicates that mitochondrial function plays an important role in the neuronal differentiation of iPS cells.
【作者單位】： 第四軍醫(yī)大學(xué)西京醫(yī)院神經(jīng)內(nèi)科;廣西醫(yī)科大學(xué)第一附屬醫(yī)院神經(jīng)內(nèi)科;挪威科技大學(xué)腫瘤研究及分子醫(yī)學(xué)部;
【基金】：國家自然科學(xué)基金(81571223)
【分類號】：R741

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