本文選題：差異表達(dá) + 轉(zhuǎn)基因　； 參考：《生物工程學(xué)報(bào)》2017年01期

【摘要】：研究已表明植物特有的一些NAC(NAM,ATAF1/2,CUC2)轉(zhuǎn)錄因子可提高植物抗逆性,利用基因芯片技術(shù)篩選轉(zhuǎn)SlNAC1基因擬南芥與野生型擬南芥間差異表達(dá)基因,能夠?yàn)檠芯哭D(zhuǎn)基因擬南芥非生物脅迫抗性相關(guān)基因提供依據(jù)。結(jié)果顯示,在轉(zhuǎn)SlNAC1基因擬南芥43 604個(gè)基因中有3 046個(gè)差異表達(dá)2倍以上的基因。對(duì)差異表達(dá)5倍以上基因經(jīng)過(guò)GO富集度統(tǒng)計(jì)學(xué)分析表明,細(xì)胞組分相關(guān)基因占33.05%;分子功能相關(guān)基因占33.95%;生物學(xué)過(guò)程相關(guān)基因占33.00%。對(duì)差異表達(dá)2倍以上基因進(jìn)行KEGG信號(hào)通路分析,結(jié)果表明有2 431個(gè)基因涉及到88個(gè)不同的信號(hào)通路。通過(guò)篩選獲得轉(zhuǎn)基因擬南芥非生物脅迫抗性相關(guān)候選基因,為后續(xù)研究NAC轉(zhuǎn)錄因子的下游基因及其調(diào)控網(wǎng)絡(luò)的構(gòu)建提供方向和理論支撐。
[Abstract]:Studies have shown that some plant specific transcription factors of NACMAMN ATAF1 / 2 CUC2 can improve plant stress resistance. Differential expression genes between transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana were screened by gene chip technique. It can provide the basis for the study of transgenic Arabidopsis Arabidopsis abiotic stress resistance related genes. The results showed that 3 046 of 43 604 genes were differentially expressed in Arabidopsis thaliana with SlNAC1 gene. Statistical analysis of differentially expressed genes by go enrichment showed that cell component related genes accounted for 33.05 genes, molecular function-related genes accounted for 33.95%, biological process genes accounted for 33.00%. The KEGG signaling pathway analysis of differentially expressed genes showed that 2 431 genes were involved in 88 different signal pathways. Candidate genes related to abiotic stress resistance of Arabidopsis thaliana were obtained by screening, which provided the direction and theoretical support for further research on downstream genes of NAC transcription factors and the construction of regulatory networks.
【作者單位】： 遼寧師范大學(xué)生命科學(xué)學(xué)院遼寧省植物生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基懶(No.31340052) 遼寧省教育廳科技研究項(xiàng)目(No.L201683655)資助~~
【分類號(hào)】：Q943.2
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本文編號(hào)：1852607