本文選題：亞麻 + LuDWF4��； 參考：《新疆大學(xué)》2017年碩士論文

【摘要】：油菜素甾醇(BRs)在調(diào)節(jié)植物細(xì)胞伸長(zhǎng),細(xì)胞壁纖維素合成等發(fā)育過程中起重要作用。亞麻(Linum usitatissimum L.)作為紡織、工業(yè)、藥用原料,經(jīng)濟(jì)價(jià)值極高。亞麻株高和纖維長(zhǎng)度決定纖維產(chǎn)量和品質(zhì)。本研究以亞麻品種“范妮”為材料,通過同源序列克隆法從亞麻葉片中獲得BR生物合成途徑中的限速酶LuDWF4,BR信號(hào)轉(zhuǎn)導(dǎo)途徑中的受體蛋白和轉(zhuǎn)錄因子Lu BRI1和LuBES1基因全序列,在野生型擬南芥Col-0和BRs受體弱突變體bril-301中進(jìn)行初步功能驗(yàn)證。同時(shí),探討了亞麻快速生長(zhǎng)期與細(xì)胞壁形成相關(guān)基因的表達(dá),為亞麻纖維發(fā)育研究奠定理論基礎(chǔ)。主要結(jié)論如下:(1)通過同源序列克隆法從亞麻葉片中克隆了LuDWF4、LuBRI1、cLuBES1和gLuBES1基因,全長(zhǎng)分別為1479 bp、3579 bp、978 bp和1428 bp。(2)成功構(gòu)建植物表達(dá)載體,分別獲得了4個(gè)(D_2,D_9,D_(10),D_(16))、3個(gè)(BR_3,BR_4,BR_(12))和3個(gè)(BE_1,BE_4,BE_(11))株系的LuDWF4、LuBRI1和gLuBES1單拷貝插入轉(zhuǎn)基因植株。(3)過表達(dá)LuDWF4基因促進(jìn)擬南芥Col-0植株增大、花期提前、花梗增高;過表達(dá)LuBRI1基因或gLuBES1基因促進(jìn)bril-301植株的生長(zhǎng)發(fā)育,挽救了bril-301矮化表型。光照和黑暗條件下,外施epi BL促進(jìn)Col-0、bril-301及LuBRI1和gLuBES1轉(zhuǎn)基因株系下胚軸伸長(zhǎng)。(4)快速生長(zhǎng)期亞麻莖韌皮纖維細(xì)胞厚度TOPMIDBOT區(qū),SP點(diǎn)下部細(xì)胞壁沒有次生加厚過程;LuBGAL3、LuBGAL5、LuBGAL6、LuBGAL9、LuCESA1、LuCESA3、LuCESA9和LuCESA10在亞麻細(xì)胞壁細(xì)胞伸長(zhǎng)過程中起作用;LuBGAL1主要促進(jìn)亞麻細(xì)胞壁加厚過程;LuSuSy和LuXTH4亞麻細(xì)胞壁發(fā)育中發(fā)揮作用。
[Abstract]:Brassin sterol (BRs) plays an important role in the regulation of plant cell elongation and cell wall cellulose synthesis. Linum usitatissimum L. As textile, industrial, medicinal raw materials, economic value is very high. Flax plant height and fiber length determine fiber yield and quality. In this study, Fanny, a flax variety, was used to obtain the receptor protein and the complete sequence of Lu BRI1 and LuBES1 genes in Br biosynthesis pathway from flax leaves by homologous sequence cloning. The function of wild-type Arabidopsis thaliana Col-0 and BRs acceptor weak mutants bril-301 was preliminarily verified. At the same time, the expression of genes related to cell wall formation in the fast growing stage of flax was discussed, which laid a theoretical foundation for the study of flax fiber development. The main conclusions are as follows: (1) the LuDWF4 (LuBRI1) cLuBES1 and gLuBES1 genes were cloned from flax leaves by homologous sequence cloning. The total length of LuDWF4 and LuBRI1 gene were 1479 BP, 3579 BP, 978 BP and 1428 BP, respectively) and the plant expression vectors were successfully constructed. Four D2D9 / D10 / D _ (10) / D _ _ _ / _ _ _ Overexpression of LuBRI1 gene or gLuBES1 gene promoted the growth and development of bril-301 plants and saved bril-301 dwarfing phenotype. In light and darkness, Application of epi BL promoted elongation of Hypocotyl of Col-0 Bril-301 and LuBRI1 and gLuBES1 transgenic Lines. 4) during the Rapid growing period, there was no secondary thickening process of the cell wall at the SP site of the thickness TOPMIDBOT zone of flax stem phloem fiber cells. There was no secondary thickening process of LuBGAL3, LuBGAL5, LuBGAL9, LuCESA1, LuCESA3, LuCESA9 and LuCESA10 in the cell wall of flax. LuBGAL1 plays an important role in the development of cell wall of flax, LuSuSy and LuXTH4.
【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q943.2;S563.2

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