本文選題：尼羅羅非魚 + NITR蛋白�。� 參考：《生物技術(shù)通報》2017年05期

【摘要】：從尼羅羅非魚脾臟組織中克隆獲得新型免疫受體(NITR,Novel immune-type receptor)基因的編碼區(qū)序列(Gen Bank登錄號:KX989509;命名為On-NITR),該基因c DNA全長1 119 bp,ORF為1 026 bp,可編碼341個氨基酸,理論分子量為37.38k D,等電點為8.28。通過NCBI BLAST比對發(fā)現(xiàn)羅非魚NITR與其他已報道的物種NITR氨基酸序列相似度為27%-46%。氨基酸序列分析顯示:On-NITR具有1個信號肽區(qū)域、2個胞外Ig-domain區(qū)、1個跨膜結(jié)構(gòu)域,以及1個胞質(zhì)尾區(qū),該胞質(zhì)尾區(qū)含有NITR典型的免疫受體酪氨酸抑制基序(ITIM)和一個ITIM類似基序itim,且具有較高的保守性。熒光定量PCR分析顯示,On-NITR在健康尼羅羅非魚組織中均有表達(dá),在腸道、皮膚、肝臟表達(dá)水平較高,在胸腺、鰓、脾臟、心臟、腦組織中的表達(dá)量較低,在頭腎組織中的表達(dá)量最低。
[Abstract]:The coding region of the novel immune receptor NITRN Novel immune-type receptor gene was cloned from spleen tissue of Tilapia niloticus. GenBank accession number: KX989509; On-NITRN; c DNA length 1 1119 BP; ORF 1 026 BP, encoding 341 amino acids. The theoretical molecular weight is 37.38 KD and the isoelectric point is 8.28. The similarity of NITR amino acid sequence between NITR and other reported species was 27-46 by NCBI BLAST comparison. Amino acid sequence analysis showed that there were one signal peptide region, two extracellular Ig-domain domains, one transmembrane domain and one cytoplasmic tail region. The cytoplasmic caudal region contains a typical NITR receptor tyrosine inhibitory motif and a ITIM similar motif itim. it is highly conserved. Fluorescence quantitative PCR analysis showed that the expression of On-NITR in the tissues of healthy Nile tilapia was higher in intestine, skin and liver, but lower in thymus, Gill, spleen, heart and brain. The lowest expression was found in the head and kidney tissues.
【作者單位】： 廣東海洋大學(xué)水產(chǎn)學(xué)院廣東省水產(chǎn)經(jīng)濟(jì)動物病原生物學(xué)及流行病學(xué)重點實驗室廣東省教育廳水產(chǎn)經(jīng)濟(jì)動物病害控制重點實驗室;
【基金】：廣東省科技計劃項目(2015A020209181) 2016年廣東海洋大學(xué)優(yōu)秀學(xué)位論文培育項目(201602)
【分類號】：S917.4

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 林苑春;葉富良;譚斌;梁妹;;溫度對尼羅羅非魚急性耐鹽性的影響[J];廣東海洋大學(xué)學(xué)報;2007年04期

2 齊紅莉;沈偉良;喬秀亭;楊廣;;飼料磷和維生素D_3含量對尼羅羅非魚磷收支的影響[J];天津師范大學(xué)學(xué)報(自然科學(xué)版);2010年02期

3 楊弘;江苑;肖煒;李大宇;祝t熈，

本文編號：1834617