發(fā)布時(shí)間：2018-05-01 13:01

  本文選題：海島棉 + TCP�。� 參考：《西北植物學(xué)報(bào)》2017年05期

【摘要】：該研究根據(jù)前期海島棉的轉(zhuǎn)錄組數(shù)據(jù),通過(guò)PCR技術(shù)從海島棉‘新海21’中克隆1個(gè)TCP基因,命名為GbTCP5,其開(kāi)放閱讀框945bp,編碼314個(gè)氨基酸,預(yù)測(cè)分子量約34.95kD,等電點(diǎn)8.41;多序列比對(duì)結(jié)果表明,GbTCP5蛋白含有特征性的TCP保守結(jié)構(gòu)域;進(jìn)化樹(shù)分析表明,GbTCP5基因與GhTCP17基因在同一分支。qPCR實(shí)驗(yàn)結(jié)果表明,GbTCP5基因在35d纖維中表達(dá)量較高,暗示其可能參與棉花纖維的次生壁合成。酵母單雜交實(shí)驗(yàn)表明,GbTCP5在SD-Trp-His-Ade缺陷培養(yǎng)基上生長(zhǎng)并且X-gal檢驗(yàn)顯藍(lán)色,說(shuō)明該基因在酵母體內(nèi)具有轉(zhuǎn)錄激活功能。
[Abstract]:In this study, based on the transcriptome data of island cotton, a TCP gene named GbTCP5 was cloned from sea island cotton 'Xinhai 21' by PCR technique. The open reading frame 945 BP encoded 314 amino acids. The predicted molecular weight was about 34.95 kD and the isoelectric point was 8.41. The results of multiple sequence alignment showed that the GbTCP5 protein contained the characteristic conserved domain of TCP, and the phylogenetic tree analysis showed that the GbTCP5 gene and the GhTCP17 gene were expressed in the same branch. QPCR results showed that the GbTCP5 gene was highly expressed in the fiber for 35 days. It may be involved in the secondary wall synthesis of cotton fiber. Yeast single hybrid experiments showed that GbTCP5 grew on SD-Trp-His-Ade deficient medium and showed blue by X-gal test, which indicated that GbTCP5 had transcriptional activation function in yeast.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)農(nóng)學(xué)院農(nóng)業(yè)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：新疆自治區(qū)青年科技創(chuàng)新人才培養(yǎng)工程優(yōu)秀青年科技人才(qn2015yx015) 新疆農(nóng)業(yè)大學(xué)研究生科研創(chuàng)新項(xiàng)目課題(XJAUGRI2016011) 國(guó)家自然科學(xué)基金(31560405)
【分類號(hào)】：Q943.2;S562
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本文編號(hào)：1829449