發(fā)布時(shí)間：2018-05-01 12:24

  本文選題：楊樹 + 卡爾文循環(huán)��； 參考：《北京林業(yè)大學(xué)學(xué)報(bào)》2016年05期

【摘要】：卡爾文循環(huán)是光合作用CO_2同化的重要途徑,在植物生長發(fā)育過程中起著重要作用。磷酸核酮糖3-差向異構(gòu)酶(RPEase:EC 5.1.3.1)是卡爾文循環(huán)再生階段的一種重要酶類。本文從速生歐美雜交黑楊NE-19中克隆得到RPEase基因,構(gòu)建植物表達(dá)載體,利用農(nóng)桿菌花序侵染法轉(zhuǎn)化野生型和突變體擬南芥,通過普通PCR檢測和綠色熒光蛋白(GFP)觀測進(jìn)一步鑒定得到Ca MV35S:PdRPE:GFP超表達(dá)株系,然后對(duì)野生型、超表達(dá)株系、突變體和回補(bǔ)株系的生理指標(biāo)進(jìn)行測定。結(jié)果顯示,超表達(dá)株系RPEase活性顯著升高(P0.05)。在正常澆水的生長條件下,超表達(dá)株系相比于其他3個(gè)株系(野生型、突變體、回補(bǔ)株系),氣孔數(shù)目減少,氣孔變大,提高了植物的水分利用效率以及凈光合速率,使得超表達(dá)株系有更好的生長優(yōu)勢(shì),積累更多的淀粉。在10 d的短期干旱條件下,超表達(dá)株系的凈光合速率和水分利用效率依然顯著高于其他3個(gè)株系(P0.01)。因此,研究表明超表達(dá)RPEase基因會(huì)提高植物生物量的積累以及對(duì)短期干旱的抵抗能力。
[Abstract]:Calvin cycle is an important pathway of photosynthesis CO_2 assimilation and plays an important role in plant growth and development. RPEase: EC 5.1.3.1) is an important enzyme in Calvin cycle regeneration. In this paper, the RPEase gene was cloned from NE-19 of American and European hybrid, and the plant expression vector was constructed. The wild type and mutant Arabidopsis thaliana were transformed by Agrobacterium tumefaciens inflorescence infection. The Ca MV35S:PdRPE:GFP overexpression lines were further identified by PCR detection and GFP observation, and the physiological indexes of wild type, overexpression lines, mutants and complementary lines were determined. The results showed that the RPEase activity of the overexpressed lines increased significantly (P 0.05). Compared with other three lines (wild type, mutants, compensatory lines), the number of stomata decreased and stomata became larger, which increased the water use efficiency (Wue) and net photosynthetic rate (PN) of the plants. So that the super-expression lines have better growth advantage and accumulate more starch. Under the condition of short-term drought for 10 days, the net photosynthetic rate and water use efficiency of the overexpressed lines were still significantly higher than those of the other three lines. Therefore, overexpression of RPEase gene can increase plant biomass accumulation and resistance to short-term drought.
【作者單位】： 北京林業(yè)大學(xué)林木育種國家工程實(shí)驗(yàn)室;
【基金】：“十二五”國家科技支撐計(jì)劃課題(2015BAD07B01) 國家自然科學(xué)基金項(xiàng)目(31270656) 北京市科學(xué)研究與研究生培養(yǎng)共建項(xiàng)目
【分類號(hào)】：S718.46
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本文編號(hào)：1829355