本文選題：水稻 + 溫度敏感型�。� 參考：《作物學(xué)報(bào)》2017年10期

【摘要】：對(duì)水稻葉色進(jìn)行表型分析與基因定位,可為圖位克隆該類(lèi)基因和研究水稻光合系統(tǒng)功能奠定基礎(chǔ)。利用甲基磺酸乙酯(ethyl methanesulfonate,EMS)誘變的方法,從秈稻品種"南京11"(簡(jiǎn)稱(chēng)NJ11)中獲得溫敏黃化突變體dy1;與野生型相比,dy1在自然環(huán)境下,從苗期至成熟期始終表現(xiàn)葉片黃化,透射電鏡顯示dy1類(lèi)囊體結(jié)構(gòu)異常;同時(shí)株高、分蘗數(shù)、結(jié)實(shí)率等農(nóng)藝性狀也表現(xiàn)出極顯著的差異;實(shí)驗(yàn)室光照培養(yǎng)箱條件下,dy1苗期在20℃下表現(xiàn)白化,在25℃表現(xiàn)黃化,在30℃下為淺綠的表型;遺傳分析表明水稻溫敏黃化突變體dy1的表型是由1對(duì)隱性基因控制;將突變體與粳稻廣親和品"02428"雜交,構(gòu)建F2群體,從中選出隱性極端個(gè)體,通過(guò)基因定位,將控制黃化的基因限定在第1染色體長(zhǎng)臂上標(biāo)記Y-4和Y-35之間的115 kb的區(qū)間內(nèi),含有16個(gè)開(kāi)放閱讀框(ORF),測(cè)序發(fā)現(xiàn),dy1中編碼尿嘧啶核苷酸激酶的基因LOC_Os01g73450的第4個(gè)內(nèi)含子與第5個(gè)外顯子交界處存在單堿基替換,擬作為候選基因;且葉綠體合成相關(guān)基因表達(dá)量顯著降低,猜測(cè)該基因可能參與了葉綠素合成途徑。
[Abstract]:The phenotypic analysis and gene mapping of rice leaf color can lay a foundation for the mapping cloning of these genes and the study of the function of rice photosynthetic system. The thermo-sensitive yellowing mutant dy1 was obtained from the indica rice variety "Nanjing 11" (NJ11) by the method of mutagenesis of ethyl methanesulfonate EMS.Compared with the wild type, it showed leaf yellowing from seedling to maturity in natural environment. Transmission electron microscopy showed that dy1 thylakoid structure was abnormal, at the same time, plant height, tiller number, seed setting rate and other agronomic traits also showed significant differences. Genetic analysis showed that the phenotype of rice thermo-sensitive yellow mutant dy1 was controlled by a pair of recessive genes, and F _ 2 population was constructed by crossing the mutant with japonica rice wide compatibility line "02428", from which the recessive extreme individuals were selected. By gene mapping, the genes controlling yellowing were limited to 115kb between Y-4 and Y-35 labeled on the long arm of chromosome 1. It was found that the fourth intron of LOC_Os01g73450, which encodes uropyrimidine nucleotide kinase, had a single base substitution at the junction of the fifth exon and the fourth intron of the nucleotide kinase encoding uropyrimidine nucleotide kinase, and it was suggested that it could be used as a candidate gene. The expression of chloroplast biosynthesis related gene was significantly decreased, suggesting that the gene might be involved in chlorophyll synthesis pathway.
【作者單位】： 南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國(guó)家重點(diǎn)實(shí)驗(yàn)室/農(nóng)業(yè)部長(zhǎng)江中下游粳稻生物學(xué)與遺傳育種重點(diǎn)實(shí)驗(yàn)室/江蘇省植物基因工程技術(shù)研究中心;中國(guó)農(nóng)業(yè)科學(xué)院;
【基金】：國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0100101-08) 江蘇省科技支撐計(jì)劃項(xiàng)目(BE2015363) 江蘇省農(nóng)業(yè)科技自主創(chuàng)新項(xiàng)目[CX(16)1029] 安徽省科技重大專(zhuān)項(xiàng)(16030701068)資助~~
【分類(lèi)號(hào)】：S511

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