本文選題：干細胞白血病基因 + Cajal樣間質(zhì)細胞　； 參考：《山東醫(yī)藥》2017年15期

【摘要】：目的觀察經(jīng)尿道灌注干細胞白血病(SCL)基因重組慢病毒的糖尿病膀胱病(DCP)豚鼠膀胱Cajal樣間質(zhì)細胞(ICC)的數(shù)量、分布及超微結(jié)構(gòu)變化。方法構(gòu)建SCL基因重組慢病毒且標定滴度。選擇雄性豚鼠70只,單次腹腔注射鏈尿佐菌素12周后構(gòu)建DCP豚鼠模型,將成功造模的27只豚鼠隨機分為實驗組、陽性對照組、空白對照組,每組9只。實驗組、陽性對照組、空白對照組豚鼠分別經(jīng)尿道灌注(轉(zhuǎn)染)SCL基因重組慢病毒、空慢病毒和等量PBS液。分別于轉(zhuǎn)染后7、14、28 d每組各處死3只豚鼠,取膀胱組織,制作切片,激光共聚焦顯微鏡下觀察GFP表達、膀胱ICC數(shù)量及分布變化,透射電鏡下觀察膀胱ICC的超微結(jié)構(gòu)變化。結(jié)果實驗組隨轉(zhuǎn)染時間延長,ICC數(shù)量逐漸增多(P均0.01),分布越發(fā)密集,但綠色熒光逐漸減退;空白組和對照組未見綠色熒光,隨轉(zhuǎn)染時間延長,ICC數(shù)量逐漸減少(P均0.05),分布稀疏;轉(zhuǎn)染14、28 d時,實驗組ICC數(shù)量較空白組和對照組增加(P均0.01)。實驗組隨轉(zhuǎn)染時間延長,ICC細胞器數(shù)量增多,形態(tài)趨于正常,細胞內(nèi)空泡減少,胞周突起延長變多,ICC超微結(jié)構(gòu)處于不斷恢復(fù)之中;而陽性對照組和空白對照組ICC超微結(jié)構(gòu)病理變化逐漸加重。結(jié)論經(jīng)尿道灌注SCL基因重組慢病毒轉(zhuǎn)染DCP豚鼠膀胱后,膀胱ICC數(shù)量增多,分布更加密集,細胞超微結(jié)構(gòu)得到修復(fù),提示SCL基因?qū)CP可能有一定治療效果。
[Abstract]:Objective to observe the number, distribution and ultrastructure of Cajal like interstitial cells (ICCs) in bladder of guinea pigs perfused with STL gene recombinant lentivirus by transurethral instillation of stem cell leukemia (SCL) gene in guinea pig bladder.Methods Recombinant lentivirus of SCL gene was constructed and its titer was calibrated.DCP guinea pig model was established by single intraperitoneal injection of streptozotocin for 12 weeks in 70 male guinea pigs. 27 guinea pigs were randomly divided into three groups: experimental group, positive control group and blank control group (n = 9 in each group).The experimental group, the positive control group and the blank control group were perfused by transurethral perfusion (transfection) of recombinant lentivirus, empty lentivirus and the same amount of PBS solution.Three guinea pigs were killed in each group on the 28th day after transfection. The bladder tissue was taken and sections were made. The expression of GFP, the number and distribution of ICC in bladder were observed under confocal laser microscope, and the ultrastructural changes of ICC in bladder were observed under transmission electron microscope.Results with the extension of transfection time, the number of ICC in the experimental group increased gradually (P < 0.01) and the distribution became denser, but the green fluorescence decreased gradually, but no green fluorescence was found in the blank group and the control group, and the number of ICC decreased gradually with the extension of the transfection time, and the distribution was sparse.On the 28th day after transfection, the number of ICC in the experimental group was higher than that in the blank group and the control group (P < 0.01).In the experimental group, the number of ICC organelles increased with the extension of transfection time, the morphology tended to be normal, the vacuoles in the cells decreased, and the ultrastructure of ICC was in the process of recovery.The ultrastructural pathological changes of ICC in the positive control group and the blank control group were gradually aggravated.Conclusion after transurethral infusion of SCL gene recombinant lentivirus into the bladder of DCP guinea pigs, the number and distribution of ICC in the bladder increased, and the ultrastructure of the cells was repaired, suggesting that SCL gene may have a therapeutic effect on DCP.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院附屬第一醫(yī)院;
【基金】：國家自然科學(xué)基金資助項目(81360120)
【分類號】：R587.2;R694

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