本文選題：轉(zhuǎn)基因甜菜 + H-品系�。� 參考：《現(xiàn)代食品科技》2017年07期

【摘要】：為實(shí)現(xiàn)轉(zhuǎn)基因甜菜品系H7-1的標(biāo)識(shí)管理和精準(zhǔn)定量,根據(jù)H7-1的5’邊界序列和甜菜谷氨酰胺合成酶基因(glutamine synthetase,GS)設(shè)計(jì)引物和探針建立雙重?cái)?shù)字PCR檢測(cè)體系。該方法的特異性、靈敏度、精密度和準(zhǔn)確度均進(jìn)行了測(cè)試。結(jié)果顯示:建立的轉(zhuǎn)基因甜菜H7-1數(shù)字PCR檢測(cè)方法特異于H7-1品系檢測(cè),在20μL反應(yīng)體中H7-1品系特異性序列和內(nèi)源基因GS的定量下限(limit of quantitation,LOQ)分別為3.1拷貝/μL和6.3拷貝/μL,檢測(cè)下限(limit of detection,LOD)檢出限分別為0.6拷貝/μL和1.3拷貝/μL,精密度和準(zhǔn)確度在可接受范圍內(nèi),該定量方法不依賴于標(biāo)準(zhǔn)曲線建立,可便捷的應(yīng)用于轉(zhuǎn)基因甜菜H7-1成分的精確定量檢測(cè)。
[Abstract]:In order to achieve the label management and accurate quantification of transgenic sugarbeet strain H7-1, a double digital PCR detection system was established by designing primers and probes based on the 5'boundary sequence of H7-1 and the sugar beet glutamine synthetase gene (GS-GS).The specificity, sensitivity, precision and accuracy of the method were tested.The results showed that the established H7-1 digital PCR detection method was specific to H7-1 strain detection.In the 20 渭 L reaction, the limit of limit of quantification of the specific sequence and the endogenous gene GS were 3.1 copies / 渭 L and 6.3 copies / 渭 L, respectively, and the detection limit of detection limit of detection LOD was 0.6 copies / 渭 L and 1.3 copies / 渭 L, respectively, and the precision and accuracy were within the acceptable range.The method does not depend on the standard curve and can be applied to the accurate quantitative detection of H7-1 components in transgenic beet.
【作者單位】： 黃埔出入境檢驗(yàn)檢疫局;廣東檢驗(yàn)檢疫技術(shù)中心廣東省動(dòng)植物與食品進(jìn)出口技術(shù)措施研究重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣東省科技計(jì)劃項(xiàng)目(2014A040401029) 出入境檢驗(yàn)檢疫行業(yè)標(biāo)準(zhǔn)計(jì)劃項(xiàng)目(2015B218k) 廣東出入境檢驗(yàn)檢疫局科技計(jì)劃項(xiàng)目(2015GDK13)
【分類號(hào)】：TS255.7
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本文編號(hào)：1759487