本文選題：油桐 + ALDH　； 參考：《經(jīng)濟(jì)林研究》2017年03期

【摘要】：為給油桐ALDH基因的結(jié)構(gòu)與功能研究提供理論依據(jù),以葡萄桐的近成熟種子為材料,根據(jù)油桐轉(zhuǎn)錄組測(cè)序結(jié)果設(shè)計(jì)引物,采用RT-PCR技術(shù)克隆了油桐ALDH22A1基因和ALDH2C4基因的全長(zhǎng)cDNA序列。ALDH22A1的cDNA序列全長(zhǎng)1 782 bp,編碼593個(gè)氨基酸,該基因編碼蛋白質(zhì)的相對(duì)分子質(zhì)量為65.60 kDa,理論等電點(diǎn)為6.62,蛋白二級(jí)結(jié)構(gòu)以α螺旋為主,具有1個(gè)明顯的跨膜結(jié)構(gòu),是穩(wěn)定的非分泌蛋白。ALDH2C4的cDNA序列全長(zhǎng)1 506 bp,編碼501個(gè)氨基酸,該基因編碼蛋白質(zhì)的相對(duì)分子質(zhì)量為54.82 kDa,理論等電點(diǎn)為6.58,蛋白二級(jí)結(jié)構(gòu)以α螺旋為主,是不具有跨膜結(jié)構(gòu)的膜外蛋白。BLASTp分析結(jié)果表明,這2個(gè)基因所編碼序列與麻瘋樹、蓖麻的乙醛脫氫酶一致性最高,高達(dá)80%以上,含有醛脫氫酶基因家族的保守結(jié)構(gòu)域。
[Abstract]:In order to provide theoretical basis for the study of the structure and function of ALDH gene, primer was designed based on the results of transcriptome sequencing from the near mature seeds of Paulownia vinifera.The full-length cDNA sequence of ALDH22A1 gene and ALDH2C4 gene. The cDNA sequence of ALDH22A1 was cloned by RT-PCR, encoding 593 amino acids.The protein encoded by this gene has a molecular weight of 65.60 kDa and a theoretical isoelectric point of 6.62. The secondary structure of the protein is 偽 -helix, with an obvious transmembrane structure. The cDNA sequence of the stable nonsecretory protein, ALDH2C4, is 1 506bp, encoding 501 amino acids.The relative molecular weight of the protein encoded by the gene is 54.82 kDa, the theoretical isoelectric point is 6.58, and the secondary structure of the protein is 偽 -helix. The results of the analysis of the extracellular protein. BLASTp without transmembrane structure indicate that the two genes encode the same sequence as Jatropha curcas.The highest consistency of acetaldehyde dehydrogenase in castor was over 80%, which contained the conserved domain of aldehyde dehydrogenase gene family.
【作者單位】： 中南林業(yè)科技大學(xué)經(jīng)濟(jì)林培育與保護(hù)省部共建教育部重點(diǎn)實(shí)驗(yàn)室;中南林業(yè)科技大學(xué)林學(xué)院;
【基金】：國(guó)家林業(yè)公益性行業(yè)科研專項(xiàng)重大項(xiàng)目(201204403)
【分類號(hào)】：S794.3
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本文編號(hào)：1746916