本文選題：番茄 + 泛素活化酶基因�。� 參考：《熱帶作物學(xué)報(bào)》2017年06期

【摘要】：泛素活化酶是蛋白泛素化所需的第一個(gè)酶,在泛素蛋白酶體途徑中發(fā)揮重要作用。本文利用生物信息學(xué)方法從番茄基因組中鑒定出2個(gè)泛素活化酶(ubiquitin activating enzyme,UBA)基因,命名為Sl UBA1和Sl UBA2。序列分析表明Sl UBA1和Sl UBA2基因的CDS序列長(zhǎng)分別為3 060和3 255 bp,分別編碼1 019和1 084個(gè)氨基酸,編碼蛋白分子量為114.15和120.46 ku,分別位于第6和9染色體。2個(gè)基因均含有5個(gè)內(nèi)含子,編碼蛋白均為酸性、疏水性蛋白;對(duì)蛋白二級(jí)結(jié)構(gòu)分析發(fā)現(xiàn)2個(gè)UBA蛋白均以α-螺旋和無規(guī)則卷曲為主;亞細(xì)胞定位預(yù)測(cè)均定位于細(xì)胞核。序列比對(duì)和進(jìn)化樹分析表明番茄UBA基因與其他物種UBA基因相似性高,進(jìn)化過程非常保守。番茄不同組織表達(dá)分析結(jié)果表明2個(gè)UBA基因在根系和花的表達(dá)量較高,果實(shí)成熟過程中的表達(dá)量相對(duì)較低。非生物脅迫試驗(yàn)結(jié)果表明:Sl UBA1基因在低溫、干旱和鹽脅迫下均上調(diào)表達(dá);而Sl UBA2基因?qū)Ψ巧锩{迫沒有明顯響應(yīng),這些結(jié)果表明Sl UBA1基因可能參與番茄對(duì)低溫、干旱和鹽脅迫的響應(yīng)。
[Abstract]:Ubiquitin activating enzyme is the first enzyme protein ubiquitination required, play an important role in the ubiquitin proteasome pathway. This method from tomato genome and identified 2 ubiquitin activating enzyme (ubiquitin activating enzyme by bioinformatics, UBA) gene, named as Sl UBA1 and Sl UBA2. sequence analysis showed that the CDS sequence of Sl UBA1 Sl and UBA2 gene were performed for 3060 and 3255 BP, respectively, encoding 1019 and 1084 amino acids, encoding a protein with molecular weight of 114.15 and 120.46 Ku, respectively, located on the sixth and ninth chromosome.2 gene contains 5 introns, encoding proteins were acidic and hydrophobic protein; protein secondary structure analysis found two of 2 UBA protein with alpha helix and random coil; subcellular localization prediction were located in the nucleus. Sequence alignment and phylogenetic analysis showed that UBA gene in tomato and other species similar to UBA gene, evolution Very conservative. The expression of different tissues of tomato analysis showed that 2 UBA genes were highly expressed in roots and flowers, the expression of fruit ripening is relatively low. Abiotic stress test results show that the Sl UBA1 gene in low temperature, drought and salt stress were up-regulated; and Sl UBA2 genes to abiotic stress no obvious response, these results suggest that Sl UBA1 gene may be involved in the response of tomato to low temperature, drought and salt stress.

【作者單位】： 中國(guó)熱帶農(nóng)業(yè)科學(xué)院南亞熱帶作物研究所;農(nóng)業(yè)部熱帶果樹生物學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：中央級(jí)公益性科研院所基本科研業(yè)務(wù)專項(xiàng)(No.1630062014010,1630062015017)
【分類號(hào)】：S641.2
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本文編號(hào)：1736882