本文選題：荸薺　切入點：淀粉　出處：《揚州大學》2017年碩士論文

【摘要】：荸莽(Eleocharis tuberosa)是莎草科荸薺屬多年生水生草本植物,原產(chǎn)于中國和印度。淀粉是荸薺球莖的主要貯藏物質(zhì),由直鏈淀粉和支鏈淀粉組成,其中直鏈淀粉可以被完全分解為麥芽糖和少量的葡萄糖,而支鏈淀粉水解后得到的產(chǎn)物為麥芽糖、葡萄糖以及分子量比較大的極限糊精。一般情況下荸薺水分和可溶性固形物含量較高,淀粉含量較低,渣少,味甜,口感較好,因此直鏈淀粉的含量及其與支鏈淀粉的比例將影響著荸莽球莖的品質(zhì)。前人研究已證明,顆粒結(jié)合型淀粉合成酶(GBSS)在直鏈淀粉合成過程中起著關鍵作用。論文以荸薺品種'紅寶石'為材料,克隆得到荸薺GBSS基因的全長cDNA序列、基因組DNA序列,并分析GBSS基因在荸薺品種'紅寶石'、'蘇州荸薺'中的表達特性。主要結(jié)果如下:1、以荸薺'紅寶石'幼嫩球莖總RNA為模板,采用RACE-PCR技術,克隆得到長為2 265 bp的荸薺GBSS cDNA序列,其開放閱讀框為1 818 bp,編碼605個氨基酸,編碼蛋白的分子量為66.5 kDa。2、以荸薺'紅寶石'幼嫩球莖為材料,提取荸薺總DNA,克隆得到長4 578 bp的GBSS基因DNA序列,包括13個外顯子和12個內(nèi)含子,其中第1內(nèi)含子最大,長504 bp,第7內(nèi)含子最小,長83 bp;第1外顯子最大,長532 bp,第5外顯子最小,長64 bp,有5個外顯子的長度小于100 bp。3、采用qPCR技術分析了荸薺GBSS的表達特性。荸薺GBSS在不同組織中的表達規(guī)律為:球莖匍匐莖葉狀莖分株芽,且球莖中GBSS表達量顯著高于其他組織,GBSS在葉狀莖及分株芽中表達量極低。在'紅寶石'和'蘇州荸薺'的球莖膨大過程中,GBSS表達量均表現(xiàn)為先上升后下降的趨勢;且GBSS在'紅寶石'球莖膨大過程中的表達量均高于'蘇州荸莽'球莖膨大各時期的表達量,膨大中期表達量達最高,匍匐莖中表達量最低。
[Abstract]:Eleocharis tuberosa is a perennial aquatic herb of water chestnut of Cyperaceae, native to China and India.Starch is the main storage material in the bulb of water chestnut, which is composed of amylose and amylopectin, in which amylose can be completely decomposed into maltose and a little glucose, and the product of amylopectin hydrolysis is maltose.Glucose and limit dextrin with higher molecular weight.In general, water content and soluble solids of water chestnut are higher, starch content is lower, residue is less, taste is sweet, taste is better, so the content of amylose and its ratio to amylopectin will affect the quality of corm.Previous studies have proved that granular-bound starch synthase (GBSS) plays a key role in amylose synthesis.In this paper, the full-length cDNA sequence and genomic DNA sequence of GBSS gene of water chestnut were cloned from the water chestnut variety 'ruby', and the expression characteristics of GBSS gene in 'ruby' Suzhou water chestnut were analyzed.The main results were as follows: 1. Using the total RNA of young bulbs of Chinese water chestnut 'ruby' as template, the GBSS cDNA sequence of 2 265 BP was cloned by using RACE-PCR technique. The open reading frame was 1 818 BP, encoding 605 amino acids.The molecular weight of the encoded protein was 66.5 kDa.2.The total GBSS of water chestnut was extracted from the young bulb of Chinese water chestnut 'ruby', and the DNA sequence of GBSS gene was cloned, including 13 exons and 12 introns, in which the first intron was the largest.The length of 504 BP, the smallest intron 7, 83 BP, the largest exon 1, 532bp, the smallest exon 5, 64 BP, and the length of 5 exons were less than 100bp.3.The expression characteristics of GBSS in water chestnut were analyzed by qPCR technique.The expression patterns of GBSS in different tissues were as follows: bulb stolon leaflike shoot ramet, and the expression of GBSS in corm was significantly higher than that in other tissues in leaf stem and ramet bud.During the bulbous enlargement of 'ruby' and 'Suzhou water chestnut', the expression of GBSS increased first and then decreased.The expression of GBSS in the process of 'ruby' bulbous expansion was higher than that in 'Suzhou' bulbous expansion stage, and the highest expression was found in the middle stage of expansion, and the lowest in stolon.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S645.3

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