本文選題：農(nóng)桿菌　切入點：轉基因棉花　出處：《中國農(nóng)業(yè)科技導報》2016年04期

【摘要】：利用農(nóng)桿菌介導法將耐旱耐鹽轉錄因子基因Pe DREB2a和Kc ERF導入受體材料陸地棉R15中,獲得12個株系的轉基因植株,通過硫酸卡那霉素初篩以及PCR分子檢測最終獲得7個轉Kc ERF-Pe DREB2a基因的棉花株系。通過測定轉基因棉花的抗逆相關生理指標以及對基因相對表達量測定分析,結果表明,200mmol/L的Na Cl和15%的PEG-6000脅迫處理后,轉基因棉花幼苗的CAT、SOD活性,游離脯氨酸含量均高于對照組,MDA含量較對照組棉花明顯下降。實時定量RT-PCR結果表明,干旱脅迫下,轉基因棉花幼苗葉片中Pe DREB2a基因的表達量高于Kc ERF基因的表達量;高鹽脅迫下,轉基因棉花葉片中的Pe DREB2a,Kc ERF基因的相對表達量持平。本研究結果表明,在干旱、高鹽脅迫下,Kc ERF-Pe DREB2a基因有助于提高棉花的耐旱耐鹽能力。
[Abstract]:Agrobacterium tumefaciens were used to transfer the drought and salt tolerant transcription factor genes Pe DREB2a and Kc ERF into Upland Cotton R15, and 12 transgenic plants were obtained.Through the primary screening of kanamycin sulfate and PCR molecular analysis, 7 cotton lines with Kc ERF-Pe DREB2a gene were obtained.By determining the physiological indexes related to stress resistance and the relative expression of genes in transgenic cotton, the results showed that the activity of CATD-SOD in transgenic cotton seedlings was increased after NaCl and 15% PEG-6000 stress.The content of free proline was significantly lower than that of control cotton.The results of real-time quantitative RT-PCR showed that, under drought stress, the expression of Pe DREB2a gene was higher than that of Kc ERF gene in transgenic cotton seedling leaves, and the relative expression of Pe DREB2a Kc ERF gene in transgenic cotton leaves was the same under high salt stress.The results showed that Kc ERF-Pe DREB2a gene was helpful to improve drought tolerance and salt tolerance of cotton under drought and high salt stress.
【作者單位】： 四川農(nóng)業(yè)大學生命科學學院;中國農(nóng)業(yè)科學院生物技術研究所;深圳市鐵漢生態(tài)環(huán)境股份有限公司;
【基金】：國家轉基因生物新品種培育重大專項(2014ZX08005-004) 廣東省省級科技計劃項目(2015B090904008)資助
【分類號】：S562
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本文編號：1702202