發(fā)布時間：2018-04-01 01:02

  本文選題：鴨　切入點(diǎn)：MITF基因　出處：《華中農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：MITF基因編碼小眼畸形相關(guān)轉(zhuǎn)錄因子,KIT基因編碼干細(xì)胞因子受體,多數(shù)文獻(xiàn)是對MITF基因、KIT基因在黑色素形成中起的重要作用及它們與動物毛色之間的關(guān)聯(lián)進(jìn)行的研究。MITF基因可調(diào)控PGC-1α(過氧化物酶體增值劑受體γ共激活劑1-α),PGC-1α能夠影響肌肉功能,如肌肉耐力,肌纖維類型轉(zhuǎn)換。該基因能夠與MAPK1互相作用,而MAPK信號途徑的活化可以進(jìn)一步增加骨骼肌纖維細(xì)胞蛋白含量,增加肌纖維長度和橫截面直徑,據(jù)此推測MITF基因?qū)∪獾纳L發(fā)育有一定的影響,而KIT基因是MITF基因的靶基因,并且可調(diào)控骨髓造血干細(xì)胞HSC,對于血細(xì)胞的正常生成具有重要作用,血細(xì)胞中的紅細(xì)胞可為肌細(xì)胞供氧,對于肌細(xì)胞的生長發(fā)育有非常重要的作用,因此推測KIT基因?qū)∪獾纳L發(fā)育也有一定的影響,肌肉的生長發(fā)育情況會直接影響家禽的屠宰、肉質(zhì)性能的優(yōu)劣,故推測MITF基因與KIT基因會在一定程度上影響家禽的屠宰、肉質(zhì)性能,但國內(nèi)外對這兩個基因與動物屠宰、肉質(zhì)性狀之間的關(guān)聯(lián)研究鮮少報道,所以本實(shí)驗(yàn)使用實(shí)驗(yàn)室現(xiàn)存的連城白鴨和白改鴨雜交后建立的F2代資源群體以及櫻桃谷鴨作為實(shí)驗(yàn)樣本,對MITF基因的11個SNPs位點(diǎn)和KIT基因的8個SNPs位點(diǎn)進(jìn)行PCR-RFLP檢測,統(tǒng)計基因分型結(jié)果,并與鴨屠宰及肉質(zhì)性狀進(jìn)行關(guān)聯(lián)分析,探討MITF、KIT基因作為影響鴨屠宰及肉質(zhì)性狀候選基因的可能性,主要研究結(jié)果如下:1.對MITF基因和KIT基因在鴨肌肉等組織中進(jìn)行表達(dá)譜分析,結(jié)果發(fā)現(xiàn)MITF和KIT基因在鴨心、肝、脾、肺、腎、胃、腿肌、胸肌中都有表達(dá);2.利用RNA-Seq的相關(guān)數(shù)據(jù),發(fā)現(xiàn)了MITF基因90多個疑似SNPs,利用其中11個SNPs進(jìn)行酶切分型,其基因型與鴨屠宰、肉質(zhì)性狀進(jìn)行關(guān)聯(lián)分析發(fā)現(xiàn):(1)與鴨肉質(zhì)性狀相關(guān)的SNP位點(diǎn)如下:與鴨腿肌纖維直徑相關(guān)的位點(diǎn)是C808002T、C808890T、C820188T、T844468C和G846879A位點(diǎn);與鴨腿肌或胸肌肉色相關(guān)的位點(diǎn)是C808890T、T810554G、C820188T、G834108T和T844865C位點(diǎn);與鴨胸肌或腿肌pH相關(guān)的位點(diǎn)是G810251T、C810282T和G834108T位點(diǎn);與鴨胸肌或腿肌失水率相關(guān)的位點(diǎn)是G810251T、C810282T、C810754A、G834108T和T844865C位點(diǎn);與鴨胸肌或腿肌熟肉率相關(guān)的位點(diǎn)是C810754A和G834108T位點(diǎn);與鴨腿肌或胸肌破碎系數(shù)相關(guān)的位點(diǎn)是G834108T和T844468C位點(diǎn);(2)與鴨屠宰性狀相關(guān)的SNP位點(diǎn)如下:與鴨屠宰率相關(guān)的位點(diǎn)是C810754A和G834108T位點(diǎn);與鴨心臟指數(shù)相關(guān)的位點(diǎn)是T810554G和T844468C位點(diǎn);與鴨肝重、肝臟指數(shù)、腹脂皮脂相關(guān)的位點(diǎn)是C810754A位點(diǎn);3.對本實(shí)驗(yàn)室前期利用混池測序在鴨KIT基因中發(fā)現(xiàn)的SNP,在資源群體中進(jìn)行了酶切分型,對基因型與鴨屠宰、肉質(zhì)性狀進(jìn)行關(guān)聯(lián)分析發(fā)現(xiàn):(1)與鴨肉質(zhì)性狀相關(guān)的SNP位點(diǎn)如下:與鴨腿肌或胸肌肉色相關(guān)的位點(diǎn)是T5168C、A1238C、G1064C、A6578G、A1221T、A5557G和C1243T位點(diǎn);與鴨胸肌或腿肌失水率相關(guān)的位點(diǎn)是G1064C位點(diǎn);與鴨胸肌熟肉率相關(guān)的位點(diǎn)是A6578G位點(diǎn);與胸肌破碎系數(shù)相關(guān)的位點(diǎn)是T5168C和A1221T位點(diǎn);(2)與鴨屠宰性狀相關(guān)的SNP位點(diǎn)如下:與鴨屠宰率相關(guān)的位點(diǎn)是T5032C和A1221T位點(diǎn);與鴨心臟指數(shù)相關(guān)的位點(diǎn)是G1064C位點(diǎn);與鴨腹脂或皮脂相關(guān)的位點(diǎn)是T5168C、A1221T、A5557G和C1243T位點(diǎn);綜上所述:MITF基因、KIT基因的SNP與鴨屠宰、肉質(zhì)性狀相關(guān),推測MITF基因、KIT基因可作為影響鴨屠宰及肉質(zhì)性狀的候選基因。
[Abstract]:The MITF gene encoding microphthalmia associated transcription factor, KIT gene encoding stem cell factor receptor, most of the literature is on the MITF gene, KIT gene in melanin formation of.MITF gene play an important role in the association between them and the color of the animal and the regulation of PGC-1 alpha (peroxidase body added receptor gamma CO activation agent 1-), alpha PGC-1 alpha can affect muscle function, such as muscle endurance, muscle fiber type conversion. The gene can interact with MAPK1, and the activation of MAPK signaling pathway can increase the protein content of skeletal muscle fibers, increase muscle fiber length and diameter of cross section, it is inferred that MITF gene has a certain influence and development for muscle growth, while the KIT gene is the target gene of MITF gene, and the regulation of bone marrow hematopoietic stem cells HSC, plays an important role in the normal production of blood cells, blood cells red blood cells for Muscle cells for oxygen, plays a very important role in muscle cell growth and development, so it has certain effects on the growth of KIT gene on muscle development, growth and development of muscle will directly affect poultry slaughtering, meat performance, suggesting that MITF gene and KIT gene can influence the slaughter of poultry in some degree meat performance, but at home and abroad on the two genes and animal slaughter, rarely reported in association studies between meat quality traits, so this experiment use the existing laboratory of Liancheng white duck and white duck changed after the establishment of the hybrid F2 generation resource group and cherry valley duck as the experimental samples, 8 SNPs loci and 11 SNPs loci and KIT the gene of MITF gene detected by PCR-RFLP genotyping results, statistics, and analysis, explore the association of MITF with duck slaughter and meat quality traits, KIT gene as the effects of duck slaughtering and meat quality trait candidate The possibility of genetic, the main results are as follows: 1. the MITF gene and KIT gene in duck muscle expression spectrum analysis, the results showed that MITF and KIT genes in duck heart, liver, spleen, lung, kidney, stomach, leg muscle, breast muscle are expressed; 2. using RNA-Seq data. MITF gene was found more than 90 suspected SNPs, using the 11 SNPs RFLP, the genotype and the duck slaughtering, meat quality traits correlation analysis found that: (1) SNP locus associated with duck meat quality traits as follows: associated with duck leg muscle fiber diameter is C808890T, C820188T loci C808002T, T844468C, and G846879A locus; associated with duck leg muscle and breast muscle color loci C808890T, T810554G, C820188T, G834108T and T844865C loci; associated with duck breast or leg muscle pH sites are G810251T, C810282T and G834108T loci; associated with duck breast or leg muscle water loss rate of site is G810251T, C8 10282T, C810754A, G834108T and T844865C loci; associated with duck breast or leg muscle rate of cooked meat loci is C810754A and G834108T sites; and duck leg muscle or muscle breaking coefficient related site is G834108T and T844468C loci; (2) SNP locus associated with duck carcass traits are as follows: the rate of related sites and duck slaughter is the C810754A and G834108T loci; associated with duck heart index is T810554G loci and T844468C loci; and the liver weight, liver index, abdominal fat sebum related site is C810754A site; 3. of the previous use of mixed pool sequencing in duck KIT gene found in SNP, the RFLP in resource group. The genotypes with duck slaughtering, meat quality traits correlation analysis found that: (1) SNP locus associated with duck meat quality traits as follows: associated with duck leg muscle and breast muscle color loci T5168C, A1238C, G1064C, A6578G, A1221T, A5557G and C1243T Point; associated with duck breast or leg muscle water loss rate of loci is G1064C loci; rate related loci and A6578G loci is the duck breast meat; related sites and chest breaking coefficient is T5168C and A1221T loci; (2) SNP locus associated with duck carcass traits are as follows: the rate of related sites and duck slaughter is T5032C and A1221T sites; associated with duck heart index locus is associated with the G1064C locus; duck abdominal fat or sebum sites are T5168C, A1221T, A5557G and C1243T loci; Summary: MITF gene, KIT gene and SNP duck slaughtering, meat quality traits, suggesting that MITF gene, KIT gene can be used as a candidate gene of duck slaughter and meat quality traits.

【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S834

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊玉靜;張丹瑾;聶瑞強(qiáng);謝建山;范瑞文;高文俊;董常生;;綿羊MITF-M在黑素細(xì)胞中過表達(dá)后的功能分析[J];中國農(nóng)業(yè)科學(xué);2016年21期

2 張浩;徐超;宋興超;周寧;張林波;邢秀梅;趙家平;;黑色素細(xì)胞調(diào)控小眼畸形相關(guān)轉(zhuǎn)錄因子的研究進(jìn)展[J];畜牧與獸醫(yī);2016年08期

3 劉安芳;胡彥競科;向博;楊旭生;馬興宇;蔡杰;;鵝H-FABP基因多態(tài)性及其與屠宰性能的相關(guān)研究[J];中國畜牧雜志;2016年13期

4 董飚;段修軍;孫國波;王麗華;卞友慶;;GH基因?qū)谟鸱嗴w重和屠宰性能的遺傳效應(yīng)分析[J];浙江農(nóng)業(yè)學(xué)報;2015年01期

5 戴國俊;孫大輝;王翔;孫明明;王金玉;張跟喜;謝愷舟;施會強(qiáng);侍苗苗;;zyxin基因外顯子7(exon7)SNP位點(diǎn)與京海黃雞屠宰及肉品質(zhì)性能的相關(guān)性[J];中國獸醫(yī)學(xué)報;2013年08期

6 韓吉龍;岳耀敬;裴杰;郭婷婷;劉建斌;郭健;孫曉萍;馮瑞林;楊博輝;;藏羊刺鼠信號蛋白基因(Agouti)的多態(tài)性及不同顏色被毛皮膚組織中Agouti與小眼畸形相關(guān)轉(zhuǎn)錄因子基因(MITF)表達(dá)的定量分析[J];農(nóng)業(yè)生物技術(shù)學(xué)報;2013年03期

7 任祥;王軍眉;潘學(xué)華;;不同品種肉兔屠宰測定報告[J];畜禽業(yè);2012年08期

8 吳嬋;李輝;李敬瑞;李謙;;貴妃雞體尺及屠宰性狀的測定與相關(guān)分析[J];河南農(nóng)業(yè)科學(xué);2011年11期

9 萬明春;霍俊宏;周泉勇;楊群;李小光;林可團(tuán);;2組不同體重的玉山黑豬屠宰測定試驗(yàn)[J];江西農(nóng)業(yè)大學(xué)學(xué)報;2010年04期

10 姜利;韓金霞;;影響雞肉質(zhì)性狀主要相關(guān)基因的研究進(jìn)展[J];家禽科學(xué);2010年08期

相關(guān)博士學(xué)位論文 前1條

1 白東義;蒙古白馬褪色表型形成機(jī)理的分子遺傳學(xué)研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2012年

相關(guān)碩士學(xué)位論文 前7條

1 彭思雪;鴨黑色素相關(guān)基因多態(tài)性及其與羽色性狀的關(guān)聯(lián)分析[D];華中農(nóng)業(yè)大學(xué);2016年

2 徐懷超;CRTC家族3個基因SNP檢測及其與秦川肉牛生長性狀和胴體性狀的關(guān)聯(lián)性分析[D];西北農(nóng)林科技大學(xué);2016年

3 翟正曉;基于RAD簡化基因組測序技術(shù)的13種中國地方優(yōu)良雞品種SNPs多態(tài)性圖譜構(gòu)建及群體遺傳學(xué)分析[D];上海交通大學(xué);2014年

4 梁正翠;鴨的色素相關(guān)基因ASIP和AGRP的研究[D];揚(yáng)州大學(xué);2010年

5 柯常育;精武優(yōu)質(zhì)肉鴨屠宰及肉質(zhì)性狀的遺傳參數(shù)估計[D];華中農(nóng)業(yè)大學(xué);2009年

6 余文敏;豬四個肌肉相關(guān)基因分離、SNPs檢測及遺傳效應(yīng)分析[D];華中農(nóng)業(yè)大學(xué);2008年

7 王聰明;豬IGF-Ⅱ基因SNPs及其與生長、胴體性狀相關(guān)性分析[D];吉林大學(xué);2008年

，

本文編號：1693244