本文選題：虎源大腸桿菌　切入點(diǎn)：喹諾酮耐藥決定區(qū)(QRDR)　出處：《黑龍江畜牧獸醫(yī)》2017年16期 　論文類型：期刊論文

【摘要】：為了解大腸桿菌對(duì)氟喹諾酮類藥物的耐藥機(jī)制和喹諾酮耐藥決定區(qū)GyrA、GyrB、ParC、ParE四種基因的流行情況,采用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)技術(shù)對(duì)30株虎源大腸桿菌的耐藥菌株進(jìn)行了氟喹諾酮類藥物耐藥基因的檢測(cè),并對(duì)目的片段進(jìn)行測(cè)序分析。結(jié)果表明:GyrA、GyrB、ParC、ParE陽(yáng)性率分別為40.00%、63.33%、63.33%、40.00%;GyrA亞基發(fā)生Ser83→Leu、Asp87→Asn、Glu214→Gly的突變,GyrB亞基發(fā)生Ser195→Asn的突變,ParC亞基上氨基酸未發(fā)生取代,ParE亞基發(fā)生Ser85→Ala的突變。說(shuō)明GyrA、GyrB亞基上發(fā)生的氨基酸替代是耐藥菌對(duì)氟喹諾酮類藥物產(chǎn)生耐藥性的主要機(jī)制之一。
[Abstract]:In order to understand the resistance mechanism of Escherichia coli to fluoroquinolones and the prevalence of four genes in GyrAgyr GyrBfus Parc Pare, a quinolone resistance determinant, Polymerase chain reaction (PCR) technique was used to detect fluoroquinolone-resistant genes in 30 strains of Escherichia coli from tiger origin, and the target fragment was sequenced. The results showed that the positive rate of Ser83 was 40.0063.3340.0063.3343. 鈫扡eu,Asp87. 鈫扐sn,Glu214. 鈫扥ccurrence of Ser195 by mutation of GyrB subunit in Gly. 鈫扵he amino acids on the mutant Parc subunit of Asn have not replaced the Pare subunit to produce Ser85. 鈫扵he mutation of Ala suggests that amino acid substitution on GyrB subunit is one of the main mechanisms of resistance of resistant bacteria to fluoroquinolones.
【作者單位】： 東北林業(yè)大學(xué)野生動(dòng)物資源學(xué)院;
【基金】：東北林業(yè)大學(xué)大學(xué)生創(chuàng)新訓(xùn)練計(jì)劃項(xiàng)目(201610225219)
【分類號(hào)】：S852.612
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本文編號(hào)：1650929