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  本文選題：抗性基因　切入點：接種污泥　出處：《環(huán)境科學與技術》2017年08期 　論文類型：期刊論文

【摘要】：近年來,抗生素抗性基因作為一種新興的環(huán)境污染物,已經(jīng)在多種水環(huán)境中被檢測出。該文研究比較了接種污泥(IS)、傳統(tǒng)活性污泥工藝(ASC)、序批式生物膜反應器(SBBR)中抗性基因的變化規(guī)律。3種系統(tǒng)均檢測出了OXA-1、OXA-2以及OXA-10 3種-內酰胺抗性基因。該文還通過實時定量PCR技術對3種抗性基因進行定量檢測。結果表明,傳統(tǒng)活性污泥工藝對β-內酰胺抗性基因去除效果更好。增加抗生素的初始濃度可以有效去除ASC及SBBR系統(tǒng)中攜帶抗性基因的抗性微生物。研究還表明,若停止對3個生物系統(tǒng)中加抗生素,并運行10個反應周期(每個周期為12 h)后,3個系統(tǒng)的抗性基因濃度均有所降低。然而,若使其在低抗生素條件下運行10個周期后,3個生物系統(tǒng)的抗性基因濃度均有所增加。
[Abstract]:In recent years, antibiotic resistance genes as a new environmental pollutant, It has been detected in a variety of water environments. In this paper, the variation of resistance genes in inoculated sludge, traditional activated sludge process and sequencing batch biofilm reactor has been studied. 3 systems have detected three kinds of OXA-1 OXA-2 and OXA-10. -lactam resistance genes. Three resistant genes were quantitatively detected by real-time quantitative PCR. The traditional activated sludge process is more effective in removing 尾 -lactam resistance genes. Increasing the initial concentration of antibiotics can effectively remove the resistant microorganisms carrying resistance genes in ASC and SBBR systems. The concentration of resistant genes in 3 biological systems decreased after the antibiotic treatment was stopped and 10 reaction cycles were performed (12 h each cycle). The concentration of resistant genes in all three biological systems increased after 10 cycles under low antibiotic conditions.
【作者單位】： 北京化工大學化學工程學院;北京市環(huán)境污染控制與資源化工程研究中心;
【基金】：國家自然科學基金項目(51178022);國家自然科學基金項目(51278022)
【分類號】：X703

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