本文選題：小麥　切入點：假禾谷鐮孢　出處：《作物學報》2017年11期 　論文類型：期刊論文

【摘要】：假禾谷鐮孢(Fusarium pseudograminearum)是我國近年新發(fā)現(xiàn)的小麥病原真菌,本研究目的是揭示植物激素信號傳導途徑中相關(guān)基因表達對假禾谷鐮孢侵染的響應。利用假禾谷鐮孢野生菌株WZ2-8A侵染小麥品種周麥24,對接種后5 d和15 d樣品進行轉(zhuǎn)錄組測序,分析差異表達基因,并對候選基因進行q RT-PCR驗證。假禾谷鐮孢侵染后,小麥幼苗生長受到明顯抑制,根長、株高、根重和地上部分鮮重均顯著降低。轉(zhuǎn)錄組分析結(jié)果表明,植物激素信號傳導途徑中有29個基因差異表達,涉及到生長素、細胞分裂素和脫落酸3種植物激素。在接種后5 d有11個差異表達基因,其中2個上調(diào),9個下調(diào);在接種后15 d共有25個差異表達基因,其中8個上調(diào),17個下調(diào)。在生長素信號傳導途徑中,生長素輸入載體AUX1差異表達,影響生長素的極性運輸,從而影響小麥根部的細胞伸長。在細胞分裂素信號傳導途徑中,起正調(diào)控作用的B-ARR上調(diào)表達,推測其促進細胞分裂素的信號傳導,從而抑制細胞分裂,與生長素協(xié)同作用,造成小麥的長勢減弱。在脫落酸途徑中,脫落酸受體PYR/PYL下調(diào)表達;起到負調(diào)控作用的PP2C相關(guān)基因均上調(diào)表達。脫落酸使植物對真菌和細菌的抗性起到負調(diào)控作用,其信號傳導途徑與茉莉酸/乙烯途徑相互拮抗,脫落酸信號傳導途徑的阻遏可能會使茉莉酸/乙烯途徑信號通路打開。q RT-PCR結(jié)果基本能夠和轉(zhuǎn)錄組測序結(jié)果相擬合,說明在假禾谷鐮孢侵染脅迫下,脫落酸的信號傳導被抑制可能是中抗品種周麥24對假禾谷鐮孢產(chǎn)生一定的抗性的生理基礎(chǔ)。
[Abstract]:Fusarium pseudograminearum (Fusarium pseudograminearum) is a new pathogen of wheat in China. The purpose of this study was to reveal the response of gene expression related to plant hormone signal transduction pathway to the infection of Fusarium graminearum. The wheat variety Zhoumai 24 was infected by WZ2-8A, and the samples were sequenced 5 and 15 days after inoculation. The differentially expressed genes were analyzed, and the candidate genes were verified by Q RT-PCR. The growth of wheat seedlings was significantly inhibited, root length, plant height, root weight and aboveground fresh weight were significantly decreased after infection by Fusarium graminearum. There were 29 differentially expressed genes in plant hormone signal transduction pathway, involving auxin, cytokinin and abscisic acid. There were 11 differentially expressed genes 5 days after inoculation, 2 of which were up-regulated and 9 were down-regulated. On the 15th day after inoculation, there were 25 differentially expressed genes, of which 8 were up-regulated and 17 down-regulated. In the auxin signaling pathway, auxin input vector AUX1 differentially expressed, which affected the polar transport of auxin. In the cytokinin signal transduction pathway, B-ARR, which plays a positive role in regulating the expression of B-ARR, can promote the signal transduction of cytokinin, thus inhibiting cell division and synergism with auxin. In the abscisic acid pathway, abscisic acid receptor PYR/PYL down-regulated the expression of PP2C related genes. Abscisic acid caused the negative regulation of plant resistance to fungi and bacteria. The inhibition of abscisic acid signal transduction pathway may make the signal pathway of jasmonic acid / ethylene pathway open. Q RT-PCR results can basically match the results of transcriptome sequencing. It was suggested that the inhibition of abscisic acid signal transduction might be the physiological basis of resistance to Fusarium graminearum in the middle resistant cultivar Zhoumai 24 under infection stress of Fusarium graminearum.
【作者單位】： 河南農(nóng)業(yè)大學/小麥玉米作物學國家重點實驗室/河南糧食作物協(xié)同創(chuàng)新中心;
【基金】：國家公益性行業(yè)(農(nóng)業(yè))科技專項經(jīng)費(201503112)資助~~
【分類號】：S435.12

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