本文選題：黃秋葵　切入點(diǎn)：葉黃素　出處：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年10期 　論文類型：期刊論文

【摘要】：ε-番茄紅素環(huán)化酶(lycopene epsilon-cyclase,LCYE)是參與類胡蘿卜素合成的關(guān)鍵酶,在類胡蘿卜素合成中發(fā)揮著重要作用。為了探究黃秋葵(Hibiscus esculentus)中LCYE基因的功能,本研究成功克隆了黃秋葵類胡蘿卜素合成途徑中下游LCYE,其cDNA全長(zhǎng)1 845 bp,具有1 605個(gè)堿基的完整開(kāi)放閱讀框(ORF),編碼534個(gè)氨基酸,理論分子量(Mw)為59.73 kD,等電點(diǎn)(pI)為6.22。黃秋葵LCYE與亞洲棉(Gossypium arboretum)、可可(Theobroma cacao)及麻風(fēng)樹(shù)(Jatropha curcas)同源蛋白的相似性均在80%以上,具高度的保守性,將基因命名為HyLCYE,GenBank登錄號(hào)為:KX257999。熒光定量分析表明,LCYE在黃秋葵成熟葉中表達(dá)最高,果實(shí)發(fā)育中以花后3 d高表達(dá)。應(yīng)用超高效液相色譜法(ultra performance liquid chromatography,UPLC)對(duì)葉黃素含量進(jìn)行分析,在不同發(fā)育時(shí)期的葉中,成熟葉中葉黃素含量最高;在果實(shí)中,以花后3 d的果實(shí)含量最高。結(jié)果表明,HyLCYE基因表達(dá)與葉黃素含量成正相關(guān)。不同基因型秋葵品種同一時(shí)期的類胡蘿卜素以綠色品種最高,紅色秋葵品種次之,白色秋葵品種總含量最低。該實(shí)驗(yàn)結(jié)果為進(jìn)一步開(kāi)發(fā)利用黃秋葵提供了科學(xué)依據(jù)。
[Abstract]:蔚 -lycopene epsilon-cyclase (LCYE) is a key enzyme involved in carotenoid synthesis and plays an important role in carotenoid synthesis. In order to explore the function of LCYE gene in Hibiscus esculentus, In this study, we cloned the middle and lower reaches of the anemone carotenoid synthesis pathway LCYE, its cDNA length was 1 845 BP, with 1 605 bases complete open reading frame, encoding 534 amino acids. The theoretical molecular weight was 59.73 KD and the isoelectric point (Pi) was 6.22. The homologous proteins of LCYE, Gossypium arboretum, Theobroma cacaoand Jatropha curcasa were all more than 80%, which were highly conserved. The gene was named HyLCYEN GenBank accession number: KX257999.The fluorescence quantitative analysis showed that the highest expression of LCYE was found in the mature leaves of Okra, and the high expression was observed 3 days after anthesis in fruit development. The content of lutein was analyzed by ultra performance liquid chromatographyof UPLC. The content of lutein in mature leaves was the highest in the leaves of different developmental stages, and in the fruit, the content of lutein was the highest in mature leaves. The results showed that the expression of HyLCYE gene was positively correlated with lutein content. The carotenoids of different genotypes of okra were the highest in the same period, followed by red anemones. The results provided scientific basis for further development and utilization of Okra.
【作者單位】： 福建省農(nóng)業(yè)科學(xué)院作物研究所/蔬菜研究中心/福建省蔬菜工程技術(shù)研究中心;
【基金】：福建省屬公益類科研院所基本科研專項(xiàng)(No.2015R1026-9) 福建省自然科學(xué)基金(No.2015J01118) 福建省農(nóng)業(yè)科學(xué)院創(chuàng)新團(tuán)隊(duì)PI項(xiàng)目(No.2016PI-40)
【分類號(hào)】：Q943.2;S649
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本文編號(hào)：1639597