本文選題：荷斯坦牛　切入點(diǎn)：牛白血病　出處：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年10期 　論文類型：期刊論文

【摘要】：牛白血病是由牛白血病病毒(Bovine leukemia virus,BLV)引起的牛(Bos taurus)、綿羊(Ovis aries)等動(dòng)物的慢性腫瘤性疾病,是影響?zhàn)B牛業(yè)發(fā)展的重要傳染病之一。為探索牛趨化因子受體1(chemokine(CX-C motif)receptor 1,CXCR1)基因編碼區(qū)(coding sequence,CDS)SNPs與牛白血病易感性之間的關(guān)系,本研究用熒光共振能量轉(zhuǎn)移-定量多聚酶鏈?zhǔn)椒磻?yīng)(fluorescence resonance energy transfer-quantitative polymerase chain reaction,FRET-qPCR)對(duì)某大型奶牛場(chǎng)866頭中國(guó)荷斯坦牛牛白血病感染情況進(jìn)行檢測(cè),同時(shí)用飛行時(shí)間質(zhì)譜法(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)對(duì)CXCR1基因編碼區(qū)CXCR1-c.642 AG、CXCR1-c.816CA、CXCR1-c.980AG和CXCR1-c.1068GA 4個(gè)SNPs位點(diǎn)進(jìn)行檢測(cè),采用Logistic回歸分析CXCR1 SNPs基因型與牛白血病易感性之間的關(guān)系,結(jié)果表明:在866頭奶牛中,患白血病的牛為42頭,陽(yáng)性率為4.85%;CXCR1-c.642 AG、CXCR1-c.816CA、CXCR1-c.980AG和CXCR1-c.1068GA 4個(gè)SNPs位點(diǎn)優(yōu)勢(shì)基因型分別為GG、CC、AA和GG型,頻率分別為0.580、0.466、0.60和0.658。CXCR1-c.980 AG位點(diǎn)對(duì)牛患白血病的相對(duì)風(fēng)險(xiǎn)影響達(dá)到顯著水平(P=0.016)。CXCR1-c.980 AG位點(diǎn)GG型基因患白血病的概率為AA型基因的5.04倍。而CXCR1-c.642 AG、CXCR1-c.816CA、CXCR1-c.1068GA和CXCR1單倍型對(duì)牛白血病易感性無顯著影響(P0.05)。CXCR1c.980AG位點(diǎn)可作為奶牛白血病抗病育種的分子標(biāo)記之一,篩選CXCR1-c.980AG位點(diǎn)AA型個(gè)體有助于降低奶牛對(duì)牛白血病的易感性并提高生產(chǎn)效率。
[Abstract]:Bovine leukemia is a chronic tumor disease in animals such as Bos taurus, sheep and other animals caused by bovine leukemia virus. In order to explore the relationship between the coding sequence of CX-C motif)receptor 1 (CXCR1) gene coding sequence and the susceptibility of bovine leukemia. In this study, fluorescence resonance energy transfer quantitative polymerase chain reaction (resonance energy transfer-quantitative polymerase chain reaction) was used to detect leukemia infection in 866 Chinese Holstein cattle in a large dairy. At the same time, matrix-assisted laser desorption/ionization time of flight mass spectrometrymetric MALDI-TOF-MSwas used to detect the CXCR1 gene coding region CXCR1-c.816CAXCR1-c.980AG and CXCR1-c.1068GA. Logistic regression analysis was used to analyze the relationship between CXCR1 SNPs genotype and susceptibility to bovine leukemia. The positive rate of CXCR1-c.642 AGXCR1-c.816CAG CXCR1-c.980 AG and CXCR1-c.1068GA 4 SNPs loci were GGCCCCAA and GG, respectively. The relative risk of bovine leukemia was significantly affected by the frequencies of 0.580, 0.466, 0.60 and 0.658.CXCR1-c.980 AG, respectively. The probability of GG type leukemia at the GG locus of CXCR1-c 980 AG was 5.04 times higher than that of AA gene. The susceptibility of CXCR1-c.642 AGCXCR1-c.816CAA and CXCR1-c.1068GA and CXCR1 haplotype to bovine leukemia was 5.04 times higher than that of AA genotype. CXCR1 c. 980AG locus has no significant effect on the disease resistance breeding of dairy cattle, and it can be used as one of the molecular markers of disease resistance breeding in dairy cattle. The screening of AA type individuals at CXCR1-c.980AG loci is helpful to reduce the susceptibility to bovine leukemia and to improve the productivity of dairy cows.
【作者單位】： 揚(yáng)州大學(xué)動(dòng)物科學(xué)與技術(shù)學(xué)院;揚(yáng)州大學(xué)獸醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(No.31372286) 江蘇高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目(PAPD) 江蘇省企業(yè)研究生工作站資助
【分類號(hào)】：S858.23

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