發(fā)布時間：2018-03-16 13:12

  本文選題：龍眼　切入點：多聚半乳糖醛酸酶基因　出處：《中國南方果樹》2017年03期 　論文類型：期刊論文

【摘要】：從龍眼中分離獲得1個多聚半乳糖醛酸酶(PG)基因,命名為DlPG1。DlPG1基因與多種物種PG基因氨基酸序列有較高的同源性,其中與同屬于無患子科的荔枝同源性最高,為95%,且與荔枝的親緣關(guān)系最近。DlPG1基因包含PG基因特有4個保守區(qū)中的3個,DlPG1屬于E進(jìn)化支PG基因家族成員。環(huán)剝摘葉處理可以顯著促進(jìn)龍眼幼果脫落,對照累積落果率為6.12%,處理為89.85%,相對落果率高峰出現(xiàn)在處理后第5天。處理的離區(qū)DlPG1基因表達(dá)量始終高于對照,且表達(dá)量高峰出現(xiàn)在處理后第4天�；虮磉_(dá)量的高峰早于相對落果率高峰出現(xiàn),據(jù)此推測,DlPG1基因可能是調(diào)控龍眼幼果脫落的關(guān)鍵基因。截至目前,DlPG1基因是首例在龍眼中發(fā)現(xiàn)的屬于E進(jìn)化支的可能與器官脫落相關(guān)的PG基因。
[Abstract]:A polygalacturonidase (DlPG1.DlPG1) gene was isolated from longan and named as DlPG1.DlPG1 gene, which has high homology with PG gene of many species, and has the highest homology with Litchi, which belongs to the same family of Litchi. DlPG1 gene is a member of PG gene family of E evolutionary branch, and the treatment of girdling leaves can significantly promote the peeling of longan young fruit, and DlPG1 gene contains three of the four conserved regions of PG gene endemic to Litchi chinensis, which belong to the PG gene family of E evolutionary branch. The cumulative fruit drop rate of the control was 6.12 and that of the treatment was 89.85. The peak of the relative fruit drop rate appeared on the 5th day after treatment. The expression of DlPG1 gene in the isolated region of the treatment was always higher than that of the control. The peak of expression appeared on the 4th day after treatment, and the peak of gene expression was earlier than the peak of relative fruit drop rate. It is suggested that DlPG1 gene may be the key gene to regulate the exfoliation of young longan fruit. So far, DlPG1 gene is the first PG gene found in longan, which belongs to the E evolutionary branch and may be related to organ exfoliation.
【作者單位】： 嶺南師范學(xué)院基礎(chǔ)教育學(xué)院;農(nóng)業(yè)部熱帶果樹生物學(xué)重點實驗室/中國熱帶農(nóng)業(yè)科學(xué)院南亞熱帶作物研究所;
【基金】：中央級科研院所基本業(yè)務(wù)費(1630062015004) 廣東省湛江市非資助科技攻關(guān)計劃項目(2016B01106) 嶺南師范學(xué)院青年項目(QL1514)資助
【分類號】：S667.2
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本文編號：1620066