本文選題：赤子愛(ài)勝蚓　切入點(diǎn)：Sox　出處：《動(dòng)物學(xué)雜志》2016年01期 　論文類(lèi)型：期刊論文

【摘要】：為了解Sox2基因在赤子愛(ài)勝蚓(Eisenia foetida)再生進(jìn)程中的作用,本研究采用c DNA末端快速擴(kuò)增(RACE)方法成功克隆并得到了完整的赤子愛(ài)勝蚓Sox2基因序列(Gen Bank登錄號(hào):KP637161),其c DNA序列全長(zhǎng)2 354 bp,其中包括367 bp的5′端非翻譯區(qū),844 bp的3′端非翻譯區(qū)和編碼380個(gè)氨基酸殘基的1 143 bp開(kāi)放閱讀框。通過(guò)實(shí)時(shí)熒光定量PCR檢測(cè)了Sox2基因在發(fā)育成熟的赤子愛(ài)勝蚓不同體段(頭部、環(huán)帶和尾部)以及在尾部體段再生進(jìn)程中的表達(dá)特征。結(jié)果顯示:Sox2在不同體段中(頭部、環(huán)帶、尾部)的表達(dá)差異不顯著。在尾部斷肢后再生進(jìn)程中,隨著時(shí)間推移,Sox2的表達(dá)量明顯上調(diào),其中,截?cái)嗪?2 h,Sox2基因表達(dá)量達(dá)到峰值,是截?cái)喑跗?0 h)的22倍。研究結(jié)果表明,Sox2基因可能與蚯蚓的再生進(jìn)程有關(guān)。
[Abstract]:In order to understand the role of Sox2 gene in the regeneration process of Eisenia foetida, In this study, the rapid amplification of c DNA terminal was used to clone and obtain the complete Sox2 gene sequence of Aisheng wormworm, Genin Bank accession number: KP637161. The c DNA sequence was 2 354 BP in length, including 367bp 5'untranslated region 3' 844bp. The untranslated region and 1 143bp open reading frame encoding 380 amino acid residues were used to detect the different body segments of the Sox2 gene in the mature wormworm (head) by real-time fluorescence quantitative PCR. The results showed that there was no significant difference in the expression of w Sox2 in different body segments (head, ring, tail). With the passage of time, the expression of Sox2 was significantly up-regulated, and the peak expression of Sox2 gene reached its peak at 12 h after truncation, which was 22 times higher than that in the early period of truncation. The results showed that the Sox2 gene might be related to the regeneration process of earthworms.
【作者單位】： 海南大學(xué)農(nóng)學(xué)院;海南大學(xué)�？谑协h(huán)境毒理學(xué)重點(diǎn)實(shí)驗(yàn)室;中國(guó)農(nóng)業(yè)大學(xué)生物學(xué)院農(nóng)業(yè)生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室;海南大學(xué)環(huán)境與植物保護(hù)學(xué)院;
【基金】：海南省普通高等學(xué)校研究生創(chuàng)新科研課題(No.S201305)
【分類(lèi)號(hào)】：Q786

【參考文獻(xiàn)】

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1 陳艷玫,姚，

本文編號(hào)：1612403