本文選題：紫蘇　切入點(diǎn)：種子油脂　出處：《中國糧油學(xué)報(bào)》2016年03期 　論文類型：期刊論文

【摘要】：為了研究種子油脂及脂肪酸生物合成機(jī)制,特別是α-亞麻酸的高水平積累,采用氣相色譜法分析7個(gè)紫蘇品種的成熟種子及晉蘇1號(hào)種子不同發(fā)育時(shí)期脂肪酸組成、含量及其動(dòng)態(tài)變化;實(shí)時(shí)熒光定量PCR檢測(cè)TAG生物合成關(guān)鍵酶基因在紫蘇不同組織及種子發(fā)育不同時(shí)期的表達(dá)特性,分析基因表達(dá)與脂肪酸及α-亞麻酸合成積累之間的關(guān)系。結(jié)果表明,紫蘇種子中不飽和脂肪酸(18∶1,18∶2,18∶3)含量占總脂肪酸90%以上,種子發(fā)育過程中,總脂肪酸含量隨著鮮重增加而不斷增加,種子形態(tài)也經(jīng)歷由多水透明狀轉(zhuǎn)變?yōu)槿榘咨蛏钭仙詈蟪蔀楹稚?隨著種子不斷發(fā)育,亞油酸含量逐漸降低,而α-亞麻酸含量不斷增加,到種子成熟時(shí)達(dá)總脂肪酸質(zhì)量的60%以上。晉蘇1號(hào)作為高含α-亞麻酸的優(yōu)質(zhì)品種,α-亞麻酸含量達(dá)456.6mg/g,亞麻酸與亞油酸之比為9∶1。Real-time PCR分析結(jié)果顯示Pf DGAT1和Pf PDAT在紫蘇莖和根系中的表達(dá)量均很低,而在葉片和種子中高量表達(dá),在種子發(fā)育不同時(shí)期,Pf DGAT1表達(dá)量呈先升高后降低的趨勢(shì),開花后30 d,表達(dá)量最高。Pf DGAT1基因超量表達(dá)之后緊接著α-亞麻酸和總油脂快速積累,表明Pf DGAT1在紫蘇α-亞麻酸和總油脂積累過程中起關(guān)鍵作用。
[Abstract]:In order to study the biosynthesis mechanism of seed oil and fatty acid, especially the high level accumulation of 偽 -linolenic acid, the fatty acid composition of mature seeds of 7 perilla varieties and Jinsu-1 seed was analyzed by gas chromatography. The expression of key enzyme genes of TAG biosynthesis in different tissues of Perilla perilla and at different stages of seed development were detected by real-time fluorescence quantitative PCR. The relationship between gene expression and synthesis and accumulation of fatty acids and 偽 -linolenic acid was analyzed. The results showed that the content of unsaturated fatty acids in perilla seeds accounted for more than 90% of total fatty acids. The content of total fatty acids increased with the increase of fresh weight, and the morphology of seeds changed from water-transparent to milky white or dark purple to brown, and the content of linoleic acid decreased gradually with the development of seeds. And the content of 偽 -linolenic acid is increasing, The content of 偽 -linolenic acid was 456.6 mg / g, and the ratio of linolenic acid to linoleic acid was 9: 1. Real-time PCR analysis showed that the ratio of PF DGAT1 and PF PDAT in perilla stem was 9: 1. Real-time PCR analysis showed that the content of 偽-linolenic acid was 456.6 mg / g. The ratio of linolenic acid to linoleic acid was 9: 1. Real-time PCR analysis. And the amount of expression in the root system is very low, However, in leaves and seeds, the expression of PF DGAT1 increased first and then decreased at different stages of seed development. At 30 days after anthesis, the highest expression of .PF DGAT1 gene was overexpressed, followed by rapid accumulation of 偽 -linolenic acid and total oil, followed by rapid accumulation of 偽 -linolenic acid and total oil. These results indicate that PF DGAT1 plays a key role in the accumulation of 偽-linolenic acid and total oil in perilla.
【作者單位】： 山西農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：國家青年科學(xué)基金(31201266)
【分類號(hào)】：S567.219

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