發(fā)布時(shí)間：2018-03-07 05:10

  本文選題：南陽黑豬　切入點(diǎn)：脂肪酸組成　出處：《黑龍江畜牧獸醫(yī)》2017年18期 　論文類型：期刊論文

【摘要】：為篩選影響南陽黑豬肉質(zhì)性狀相關(guān)基因,試驗(yàn)以肌內(nèi)脂肪含量、脂肪酸含量及組成為指標(biāo),利用基因芯片技術(shù)對(duì)南陽黑豬和大白豬指標(biāo)差異極顯著個(gè)體進(jìn)行轉(zhuǎn)錄分析。結(jié)果表明:南陽黑豬單不飽和脂肪酸、多不飽和脂肪酸、飽和脂肪酸、不飽和脂肪酸、必需脂肪酸、共軛亞油酸和肌內(nèi)脂肪含量顯著或極顯著高于大白豬(P0.05或P0.01),共發(fā)現(xiàn)了148個(gè)與脂肪性狀相關(guān)的差異基因,其中起上調(diào)作用的共65個(gè),起下調(diào)作用的共83個(gè);GO分類顯示,差異基因主要參與脂肪酸合成、脂類代謝的激活、胰島素信號(hào)通路、PPAR信號(hào)通路、MAPK信號(hào)通路等眾多與脂肪沉積相關(guān)的生物學(xué)過程。
[Abstract]:In order to screen genes related to meat quality traits of Nanyang Black Pig, the content of intramuscular fat, fatty acid and composition were used as indexes. Transcriptional analysis of Nanyang Black Pig and Big White Pig was carried out by gene chip technique. The results showed that: monounsaturated fatty acid, polyunsaturated fatty acid, saturated fatty acid, unsaturated fatty acid, essential fatty acid, and essential fatty acid were analyzed in Nanyang Black Pig. The content of conjugated linoleic acid and intramuscular fat was significantly or extremely higher than that of large white pigs (P0.05 or P0.01). A total of 148 differentially related genes related to fat traits were found, of which 65 were up-regulated and 83 were down-regulated. Differential genes are involved in fatty acid synthesis, lipid metabolism activation, insulin signaling pathway, PPAR signal pathway, MAPK signal pathway and many other biological processes related to fat deposition.
【作者單位】： 南陽師范學(xué)院生命科學(xué)與技術(shù)學(xué)院;河南科技學(xué)院食品學(xué)院;內(nèi)鄉(xiāng)順發(fā)南陽黑豬保護(hù)科技有限公司;
【基金】：河南省自然科學(xué)基金項(xiàng)目(162300410205) 河南省科技攻關(guān)項(xiàng)目(162102310598)
【分類號(hào)】：S828

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