胡楊CBF基因家族的鑒定及表達特性分析
發(fā)布時間:2018-03-05 17:03
本文選題:胡楊 切入點:CBF 出處:《分子植物育種》2017年02期 論文類型:期刊論文
【摘要】:CBF(C-repeat binding factor)基因家族是AP2轉(zhuǎn)錄因子家族的一類成員,在植物抵御低溫脅迫與提高抗寒性中發(fā)揮著重要作用。為探究胡楊CBF基因家族的特性,本研究在胡楊基因組水平上共搜索到8個PeuCBFs成員,分布于6條scaffolds上,其中PeuCBF1和PeuCBF5的氨基酸和編碼序列相同。對PeuCBFs在胡楊8個組織和5種非生物脅迫下的表達特性進行分析,發(fā)現(xiàn)PeuCBFs主要在幼葉或雌雄花序中表達;PeuCBFs的表達受低溫脅迫的顯著誘導,同時受鹽、干旱和ABA脅迫誘導,但是受高溫脅迫抑制。研究結(jié)果概括了胡楊CBF基因家族的基本特性,為進一步解析PeuCBFs的功能提供了參考。
[Abstract]:The CBF(C-repeat binding factor gene family is a member of the AP2 transcription factor family, which plays an important role in plant resistance to cold stress and cold resistance. In order to explore the characteristics of CBF gene family of Populus euphratica, In this study, eight PeuCBFs members were found at the genomic level of Populus euphratica and distributed on 6 scaffolds. The amino acids and coding sequences of PeuCBF1 and PeuCBF5 were the same. The expression characteristics of PeuCBFs in 8 tissues and 5 abiotic stresses of Populus euphratica were analyzed. It was found that the expression of PeuCBFs was mainly induced by low temperature stress, salt stress, drought stress and ABA stress, but inhibited by high temperature stress in young leaves or male and female inflorescences. The basic characteristics of CBF gene family of Populus euphratica were summarized. It provides a reference for further parsing the function of PeuCBFs.
【作者單位】: 南京林業(yè)大學南方現(xiàn)代林業(yè)協(xié)同創(chuàng)新中心;林木遺傳育種國家重點實驗室中國林業(yè)科學研究院林業(yè)研究所;新疆瑪納斯縣林業(yè)局;
【基金】:林業(yè)公益性行業(yè)科研專項經(jīng)費(201404101) 中央級公益性科研院所基本科研業(yè)務(wù)費專項資金(CAFYBB20-14ZX001-4) 新疆自治區(qū)高層次人才引進工程(2014)共同資助
【分類號】:S792.11;Q943.2
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